Sentences with phrase «dead cells excluded»
The cell population of interest was determined and dead cells excluded using forward and side scatter parameters.
https://biomedical-engineering-online.biomedcentral.com/ Not exact matches
Dead cells were excluded by PI staining (Sigma, 1:3,000).
Following washes in PBS, cells were resuspended in 500 µl FACs buffer (1 × PBS, 10 % FCS) and 7AAD solution (BD Pharmingen, 5 µl / 1 × 106 cells) to exclude dead cells.