Sentences with phrase «deaminase convert»

In this assay SAHH (S - adenosylhomocysteine hydrolase) and adenosine deaminase convert the methyltransferase reaction product (S - adenosylhomocysteine) to homocysteine and inosine.

Bacteria that produce the enzyme cytidine deaminase converted the drug to an inactive form.

Not exact matches

This domain has been hypothesized to provide the enzymatic activity needed to convert C to U because it carries motifs characteristic of cytidine deaminases.

Liu and coworkers developed last year's base editor by combining three proteins: a cytidine deaminase, a natural enzyme that converts C to uridine (U); a mutated Cas9 CRISPR enzyme that doesn't cut DNA but uses an associated guide RNA to target specific DNA sequences; and a protein that prevents reversion of U back to C.

Liu and coworkers solved that problem by using directed evolution and enzyme engineering to convert a bacterial adenosine deaminase that normally works in RNA into a deoxyadenosine deaminase that converts A to inosine (I) in DNA.

The researchers use a conjugate of the deoxyadenosine deaminase with a catalytically impaired Cas9 to convert A to I at a target site and to nick the opposite strand.