Sentences with phrase «deaminase converted»

Bacteria that produce the enzyme cytidine deaminase converted the drug to an inactive form.

In this assay SAHH (S - adenosylhomocysteine hydrolase) and adenosine deaminase convert the methyltransferase reaction product (S - adenosylhomocysteine) to homocysteine and inosine.

Not exact matches

This domain has been hypothesized to provide the enzymatic activity needed to convert C to U because it carries motifs characteristic of cytidine deaminases.

Liu and coworkers developed last year's base editor by combining three proteins: a cytidine deaminase, a natural enzyme that converts C to uridine (U); a mutated Cas9 CRISPR enzyme that doesn't cut DNA but uses an associated guide RNA to target specific DNA sequences; and a protein that prevents reversion of U back to C.

Liu and coworkers solved that problem by using directed evolution and enzyme engineering to convert a bacterial adenosine deaminase that normally works in RNA into a deoxyadenosine deaminase that converts A to inosine (I) in DNA.

The researchers use a conjugate of the deoxyadenosine deaminase with a catalytically impaired Cas9 to convert A to I at a target site and to nick the opposite strand.