Sentences with phrase «deaminase from»

The assay mixtures were prepared in 25 mM potassium phosphate buffer pH 7.5, 1 mM EDTA, 2 mM MgCl2, 0.01 % Triton X-100 with 5 µM SAHH, 0.3 U / ml of adenosine deaminase from Sigma, 25 µM SAM, and 15 µM Thioglo - 1.

Not exact matches

Base editors borrow from CRISPR's components — guide RNAs (gRNAs) and Cas9 or other nucleases — but don't cut the double helix and instead chemically alter single bases with deaminase enzymes such as TadA and ADAR.

The point mutation was induced by forming a synthetic complex through removal of nuclease activity from the CRISPR system — a technique using artificial nuclease — and addition of deaminase, a deaminizing (base - modifying) enzyme, and then expressing it in yeasts and mammalian cells.

Crystal structure of an ADP - ribosylated protein with a cytidine deaminase - like fold, but unknown function (TM1506), from Thermotoga maritima at 2.70 A resolution.