Sentences with phrase «deaminase with»
The researchers use a conjugate of the deoxyadenosine deaminase with a catalytically impaired Cas9 to convert A to I at a target site and to nick the opposite strand.
https://cen.acs.org/ Not exact matches
Babies born with the defect are unable to produce an enzyme called adenosine deaminase (ADA).
They subsequently co-encapsulated the cells with magnetic iron oxide in immunoprotective alginate microcapsules and then remotely triggered cytosine deaminase expression by alternating magnetic field - induced hyperthermia.
Base editors borrow from CRISPR's components — guide RNAs (gRNAs) and Cas9 or other nucleases — but don't cut the double helix and instead chemically alter single bases with deaminase enzymes such as TadA and ADAR.
The assay mixtures were prepared in 25 mM potassium phosphate buffer pH 7.5, 1 mM EDTA, 2 mM MgCl2, 0.01 % Triton X-100 with 5 µM SAHH, 0.3 U / ml of adenosine deaminase from Sigma, 25 µM SAM, and 15 µM Thioglo - 1.
Crystal structure of an ADP - ribosylated protein with a cytidine deaminase - like fold, but unknown function (TM1506), from Thermotoga maritima at 2.70 A resolution.