Sentences with word «deamination»
Based on the nucleotide context, the authors suggest that a new mutational mechanism may be at work involving cytosine deamination of single - stranded DNA (presumably during replication).
massgenomics.org
The SureSeq mix increases the success and accuracy of hybridization - based NGS for FFPE samples by repairing damage such as nicks and gaps, oxidized bases, blocked 3» ends, and deamination of cytosine to uracil.
Gluconeogenesis from protein requires deamination, and the nitrogen molecule is then used to manufacture ammonia and urea that are both poisonous.
The only limiting factor here becomes the amount of ammonia produced by deamination.
The latter is important to understand, as deamination (the process by which the amino group is removed) creates two products: Ammonia and a carbon skeleton (Acetyl CoA / acetoacetate or pyruvate).
Cytosine deamination rates and fragmentation patterns were plotted using mapDamage2.0 (70), based on 100,000 randomly selected alignments against EquCab2.0.
«When you prepare the cell lysate and [perform] DNA denaturation, it introduces an artifact called cytosine deamination that's due to the elevated temperature [step of the protocol],» says Joyce Peng, global marketing director for Novogene in Chula Vista, California.
Depending on conditions, however, RNA and DNA have very different abilities to withstand chemical changes like depurination, deamination, and hydrolysis.