Sentences with phrase «decreased cell viability»

In contrast, ActD (≤ 100 nM) dose - dependently decreased cell viability and increased active p53 in the HepG2 cells.

Treatment with ActD at low doses of 3 - 30 nM for the 293 and 293T cells, and 3 - 100 nM for the HepG2 cells, for 24 and 48 hours dose - dependently decreased cell viability (Fig. 8).

However, treatment with ActD of 30 - 300 nM for the 293 and 293T cells, and 100 and 300 nM for the HepG2 cells, for 24 and 48 hours significantly decreased cell viability by a similar level.

Our findings confirm previously published data as treatment of Karpas 299 and SU - DHL1 cells with increasing concentrations of LY294002 led to decreased cell viability and increased apoptosis, as determined by Annexin V staining, in a dose - dependent manner (Supplementary Fig.

Similarly, silencing of mTOR also resulted in decreased cell viability and increased Annexin V — positive cells in comparison with control cells and with cells transfected with 4E - BP1 - specific siRNA (Fig. 6B).

High doses (≥ 30 nM) of ActD did not further decrease cell viability or increase p53 expression.

Not exact matches

Using a zebrafish embryo xenograft model we also demonstrated that anti-CD90 monoclonal antibodies decreased the viability and metastatic potential of insulinoma cells, suggesting that anti-CD90 monoclonals form a potential novel adjutant therapeutic modality.

For rare cell populations, these losses in recovery and viability present challenges for end users performing research on a process with potential for transfer to clinical scale by decreasing the ability for process time and cost efficiency.

Nevertheless, we examined whether the reduction of IL - 22 production by ILC3s correlated with a decrease in cell viability.

Moreover, annexin V / propidium iodide staining showed that VPA only marginally decreased the viability of in vitro — differentiated Th1 and Th17 cells at a relatively high concentration (1 mM)(Supplemental Fig. 4A, 4B), whereas VPA readily inhibited the differentiation of Th1 and Th17 cells at a low concentration (0.2 — 0.5 mM), indicating that apoptosis induction was not the primary mechanism for Th1 and Th17 cell differentiation inhibition (28, 29).

This dichotomy points out the importance of an indirect influence on proteins whose altered function is required during carcinogenesis but are too essential to be mutated (i.e., mutation of their encoding genes could decrease the viability of cancer cells).

Genetic ablation of a Na + / H + exchanger (NHE2), which is expressed in the stomach at high levels, leads to a decrease in gastric acid secretion, along with decreased viability of parietal cells, severe metaplasia, and hyperplasia of gastric mucosa.