Deep sequencing of cerebral white matter - derived RNA from the HIV (n = 4) and ODC (n = 4) patients and SURG (n = 2) groups revealed bacterially - encoded 16 s RNA sequences in all brain specimens with α - proteobacteria representing over 70 % of bacterial sequences while the other 30 % of bacterial classes varied widely.
ChIP - Seq (ChIP followed by
deep sequencing of DNA) is an extension of ChIP technology to determine the chromatin enrichment of a transcription factor on a genome - wide scale.
Deep sequencing of RNA extracts from the re-extracted archival VP62 (2012) tissue was also performed.
We assayed (1) the absolute number of 16S rRNA gene copies and (2) overall community structure queried by
deep sequencing of 16S rRNA gene tags.
has developed a practical, computational technique, IgDiscover, that enables rapid production of individualized V gene databases following
deep sequencing of expressed antibody repertoires.
To find a solution to this, Marcel Martin at Bioinformatics Long - term Support (WABI) at SciLifeLab, in collaboration with the group of Gunilla Karlsson Hedestam at Karolinska Institutet, has developed a practical, computational technique, IgDiscover, that enables rapid production of individualized V gene databases following
deep sequencing of expressed antibody repertoires.
Jacob A. Tennessen et al., «Evolution and Functional Impact of Rare Coding Variations from
Deep Sequencing of Human Exomes,» Science, Vol.
Deep Sequencing of Influenza A Virus from a Human Challenge Study Reveals a Selective Bottleneck and Only Limited Intrahost Genetic Diversification.
To facilitate whole - genome sequencing of TMAdV,
deep sequencing of a lung swab from one affected titi monkey and lung tissue from another affected monkey was performed.
To gain greater understanding of how the llama immunizations worked,
deep sequencing of the HIV binding region of the antibodies was performed.
Deep sequencing of the VHH phage libraries generated from a set of llamas, which received two different immunization protocols, showed that the new VHH and the previously described anti-HIV VHH J3 [26] were induced by immunization (Table 1).
Deep sequencing analysis has shown that this is highly unlikely in the case of J3 as no sequences were generated by
deep sequencing of the seven naïve llamas or three other immunized llamas that share greater identity with J3 than its putative germ line V gene.
Deep sequencing of isolates taken from a single patient carrying MRSA for the longest period revealed that although all isolates were of the same clade, there were small genetic differences between them, suggesting bacterial diversity within a single carrier.
Targeted
deep sequencing of other mutants also showed no off - target mutation»
«
Deep sequencing of viruses like HSV - 1 will provide a better view of the viral genetic diversity that individuals harbor, and will provide valuable information about how that influences the course of disease.»
Excavation revealed
a deep sequence of deposits containing the elephant remains, along with numerous flint tools and a range of other species such as; wild aurochs, extinct forms of rhinoceros and lion, Barbary macaque, beaver, rabbit, various forms of vole and shrew, and a diverse assemblage of snails.
Not exact matches
For example, if founders have established a company to tackle problems related to DNA
sequencing yet prior experience is in social media, a
deeper look into the team's motivations and understanding
of its target market would be warranted.
How far this creativity can go in creating the human level in the case
of any one individual depends partly upon his innate capacity but most
of all upon two other features: (1) how wide and
deep is the volume
of history that reaches him, that is, how abundant and coherent are the values that have been accumulated in the history he inherits and (2) how
deep is the communion he is able to have with other persons who embody these meanings accumulated through a long
sequence of generations.
Each sleep cycle is a
sequence of sleep stages, beginning with relatively brief, light stages
of sleep, progressing through stages
of deep sleep, and ending with REM (rapid - eye movement) sleep, the sleep state associated with dreams.
In fact, Free Birth is about women taking full responsibility for our experiences, and re-wilding herself into a
deep relationship
of trust and autonomy, allowing the physiological and biological
sequence of natural mammalian labor to unfold, undisturbed.
Researchers
sequenced nuclear DNA from three molars from layer 11 and a child's molar from a
deeper layer, 22, according to a talk by graduate student Viviane Slon, who works in the lab
of paleogeneticist Svante Pääbo at the Max Planck Institute for Evolutionary Anthropology.
To find out more about how they manage to survive, Brandon Briggs at Miami University in Oxford, Ohio, and Frederick Colwell at Oregon State University in Corvallis have
sequenced and compared genomes belonging to one particular class
of deep life — Firmicutes bacteria — sampled 21, 40 and 554 metres below the floor
of the Andaman Sea, west
of Thailand.
That's why he and hundreds
of other scientists around the world have joined Earthtime, a 10 - year endeavor to nail down the
sequence of past events on Earth by refining scientists» techniques for measuring
deep time.
«This is a story that couldn't have happened five years ago, but with the evolution
of deep sequencing, we were able to use the technology at just the right time and harness it with what we've been working on for decades,» he says.
Deep -
sequence coverage allowed identification
of 263 iSNVs (73 nonsynonymous, 108 synonymous, 70 noncoding, and 12 frameshift) in the Sierra Leone patients (6).
But the benefits
of this technology for discerning the circuits
of the mind go much
deeper, because the virus that carries the photoreceptor genes can also carry promoter
sequences that express their payload only in neurons with the appropriate molecular address.
«
Deep sequencing technology is now becoming a staple
of scientific research.
In this study that used
deep DNA
sequencing of bacterial specific genes, the guts
of both males and females exposed to lead had all
of the similar complexity in microbiota as those not exposed.
Roswell Park Cancer Institute (RPCI) researchers used
deep T - cell receptor (TCR)
sequencing to evaluate the clonal composition
of TILs and identify novel prognostic biomarkers in ovarian cancer.
This «
deep sequencing» technique has been used to characterize mixtures
of microbes living in environments such as oceans and animal guts.
«The microbiome is incredibly complex and has been the subject
of intense study in the last few years, due to the development
of deep sequencing and most importantly, due to the ability to analyze this complex data,» said Patterson.
Described in the January 7th issue
of Neuron, the technique uses «
deep,» highly sensitive whole - genome
sequencing of single neurons and a new technology that identifies inserted bits
of DNA caused by retrotransposons, one
of several kinds
of so - called somatic mutations that can arise as the brain develops.
That began to change last year with the discovery
of DNA
sequences for an organism that no one has ever actually seen living near a
deep - sea vent on the ocean floor.
In a simulation
of two colliding galaxies (
sequence, top to bottom), a central quasar blows gas
deep into space.
Understanding how a particular DNA
sequence gives rise to a particular protein provides us with some insight into that protein, but a
deeper investigation
of how the protein is made, where it is located, and how much
of it is present in different cell types is required to enable a true understanding
of its function.
As Venkateswaran and colleagues explain online today in Microbiome, they relied on state -
of - the - art «
deep -
sequencing» machines, which are better able to assess diversity and abundance
of organisms than traditional techniques.
This study was based on DNA
sequence and
deep phenotypic data from the Simons Simplex Collection, a set
of 2,760 families that have a single child affected by ASD.
In light
of these findings and since tRNAs are repeat
sequences themselves, the team had to devise a complex and stringent scheme with which they mapped the
deep sequencing reads.
Dr Nick Lane (UCL Biosciences) who led the study said, «I find this work just beautiful — it constrains a
sequence of steps going from the strange cell that seems to have been the ancestor
of all life today, right through to the
deep division between modern cells.
If we could get all
of that material out
of the
deep freeze and run it through the
sequencing machine, we would find the answer.
A team
of Spanish researchers, coordinated by the Spanish National Research Council (CSIC), has started to
sequence the genome
of the global
deep ocean.
«Researchers
sequence the genome
of global
deep ocean.»
Piero Carninci at the RIKEN Yokohama Institute's Omics Science Center in Japan, and colleagues, combined powerful computer modelling with «
deep sequencing» — a process which decodes huge numbers
of DNA
sequences to discover...
Here they photographed organisms from
deep - water samples and used technology to
sequence the genomes
of these single - celled organisms.
Specifically, 60 %
of the bacterial species
of the
deep ocean detected by massive
sequencing techniques are unknown.
The works
of sequencing (framed in the Malaspinomics project) focus on the viruses, bacteria and protists that inhabit the ocean to 4,000 meters
deep.
The technique, called ultra-rapid
deep sequencing, combines
deep sequencing — an emerging technology that reconstructs an entire DNA
sequence from a tiny snippet
of DNA — with advanced computational techniques and algorithms developed in the laboratories
of Chiu and his research collaborators.
«Tim and I were able to work very closely in all the steps
of the project from the experimental design in the wet lab to the final analysis
of the results; the major challenge was how to handle the very large number
of barley samples and for this we designed a novel approach to
sequencing that exploited
deep results in combinatorics,» Lonardi said.
A study
of this scope is possible thanks to innovative techniques for in vitro selection (such as mRNA display), as well as a revolutionary technology permitting massively parallel RNA
sequencing (known as
deep sequencing), which provides unprecedented speed and read accuracy.
«Our combined analysis
of cell - free DNA and white blood cell DNA allows for identification
of tumor DNA with much higher sensitivity, and
deep sequencing also helps us find those rare tumor DNA fragments.»