Both GpNLuc and OgNLuc BRET reporters produced bright signals without exogenous excitation and with OgNLuc being the brightest since it had an emission near 600 nm — a clear demonstration of why a BRET reporter can improve
deep tissue imaging.
As the intent is to non-toxically label live cells for whole body and
deep tissue imaging, multimerization should not be an issue.
The BX51WI is the ideal instrument for electrophysiological experiments, particularly when an excellent optical system for
deep tissue imaging is required.
Not exact matches
Dr. Gradinaru's research interests focus on developing tools and methods for neuroscience (optogenetic actuators and sensors;
tissue clearing and
imaging) as well as on investigating the mechanisms underlying
deep brain stimulation (DBS) and on the long - term effects of DBS on neuronal health, function, and ultimately behavior.
However, such multimerization capabilities should not interfere with the intended goal of non-toxically labeling cells for
deep tissue and intravital
imaging as the Amrose variants 1 — 3 were developed in vertebrate cells.
For quantifying the performance of Amrose in
deep tissue optical
imaging experiments, DT40 cells expressing no FP (control), Amrose v1 and mRaspberry were used.
Principal investigator Wen Wee Ma, MD, of Roswell Park's Phase One and Gastrointestinal Cancer Programs, explains: «Positron emission tomography — PET — is a standard tool in the clinic for
imaging tissues deep inside the body.