Sentences with phrase «derived cells in culture»

Added to patient - derived cells in culture, rapamycin enabled the development of more Purkinje precursor cells, improved the functioning of their synapses and increased their tendency to fire.

Not exact matches

Since the algae reproduces by cell division about once per day in the laboratory, numerous genetically identical cultures could be derived from the isolate.

When the Stanford team measured pyrophosphate levels in cultured cells derived from ank and normal mice, they found that the chemical accumulated in cells from the ank mice but decreased in the culture medium.

In a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collageIn a previous related study published in the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagein the Journal of Materials Science: Materials in Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagein Medicine, the same team of NTU scientists found that fish scale - derived collagen would induce human umbilical vein endothelial cells to express 2.5 times more of a specific type of collagen responsible for blood vessel formation, as compared to endothelial cells cultured on bovine collagen.

They found that indeed, they do, and that stimulating these cells led them to kill cells infected with HIV - 1 derived from latently infected cells, both in culture and in mice engineered to have a human immune system.

In BRIC 17 - 1, cell cultures derived from thale cress plants are grown in Petri dishes and later examined to determine which genes are involved in certain cellular changeIn BRIC 17 - 1, cell cultures derived from thale cress plants are grown in Petri dishes and later examined to determine which genes are involved in certain cellular changein Petri dishes and later examined to determine which genes are involved in certain cellular changein certain cellular changes.

Grima also observed this same clumping of Huntingtin protein with RanGAP1 and nuclear pore proteins to the wrong place in the cell in brain tissue and cultured brain cells derived from deceased patients with Huntington's disease.

In an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channeIn an effort to overcome these limitations, a team at the Wyss Institute for Biologically Inspired Engineering led by its Founding Director, Donald Ingber, M.D., Ph.D., had previously engineered a microfluidic «Organ - on - a-Chip» (Organ Chip) culture device in which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channein which cells from a human intestinal cell line originally isolated from a tumor were cultured in one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channein one of two parallel running channels, separated by a porous matrix - coated membrane from human blood vessel - derived endothelial cells in the adjacent channein the adjacent channel.

Using cultured cells derived from human tumors of the breast and prostate gland, they confirmed that the IL6R / STAT3 / miR -34 a feedback loop is also activated in other tumor types.

Further testing of this culture method on kidney progenitor cells derived from human iPS cells resulted in four times the total amount of cells over 8 days.

Testing many variants, the team eventually found an artificial receptor design that worked well in cell culture, enabling host T cells to efficiently destroy cells producing antibodies to desmoglein, including those derived from PV patients.

Researchers used cultured stem cells derived from their feline patients in the hope of reducing inflammation and promoting tissue regeneration.

Although a clause in the law that funds NIH prevents the agency from funding research that would harm or destroy an embryo, a lawyer at the Department of Health and Human Services ruled in 1999 that because stem cells — which can grow ad infinitum in culture — are not themselves embryos, the NIH could fund work with cells that were derived by privately funded researchers or researchers overseas.

In this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.&raquIn this tissue system, we can culture patient - derived megakaryocytes — the bone marrow cells that make platelets — and also endothelial cells, which are found in bone marrow and promote platelet production, to design patient - specific drug administration regimes.&raquin bone marrow and promote platelet production, to design patient - specific drug administration regimes.»

Scientists at the University of Luxembourg have succeeded in turning human stem cells derived from skin samples into tiny, 3 - D, brain - like cultures that behave very similarly to cells in the human midbrain.

When BMP protein was added for three days in human pluripotent stem cell - derived gut tube cultures, it induced a posterior HOX code.

The percentage of cells expressing both E-selectin and VCAM - 1 (or DiI - Ac - LDL for bone - derived cultures) after 5 h of TNF - α stimulation in the second series of selection increased significantly to reach values of 30 — 40 % for most cultures.

However, chondrogenesis assays in chick micromass cultures showed that enhanced signaling by the mutant R206H ACVR1 can be partially inhibited by the BMP antagonist Noggin, suggesting that in addition to BMP - independent activity by the R206H ACVR1 receptor, this mutant receptor is also responsive to BMP, consistent with previous BMP signaling pathway studies that used cells derived from FOP patients (48 — 50).

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using our novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Glial cell line - derived neurotrophic factor promotes the survival of early postnatal spinal motor neurons in the lateral and medial motor columns in slice culture.

Human iPS cell - derived hepatocytes differentiated with our robust differentiation protocol and cultured using a novel maintenance medium provide an inexhaustible, consistent supply of functional hepatocytes that can be used to advance the understanding of diseases related to dysfunction in liver metabolism, including NAFLD / NASH, type 2 diabetes, and metabolic syndrome.

Differential osteogenicity of multiple donor - derived human mesenchymal stem cells and osteoblasts in monolayer, scaffold - based 3D culture and in vivo.

Suspension culture of hepatocyte - derived reporter cells in presence of albumin to form stable three - dimensional spheroids.

To ensure that any pathology developed in the cultured neurons could be attributed solely to the genetic defect, the researchers also derived control neurons from iPS cells in which the mutation had been corrected.

Delaney et al. [4] who have shown that cord blood - derived CD34 + cells cultured in StemSpan ™ medium with cytokines, on plates coated with Notch ligand, are expanded ex vivo while still maintaining their ability to engraft into immunocompromised mice.

Bone marrow - derived mesenchymal stem cells (BM - MSCs) are obtained by isolating mononucleated cells from a marrow aspirate by centrifugation on a density gradient, followed by adherent cell expansion in tissue culture.

For this purpose, at least 30 different cell lines derived from biopsies (HNSCC — head and neck cancer — patients) wil be collected and grown in culture to esstablish cell lines.

Defining Adipose Tissue - Derived Stem Cells in Tissue and in Culture (June, 2010) in Histology Histopathology.

These compounds also corrected SMN2 RNA splicing and increased SMN protein levels in cell cultures obtained from SMA patients, including stem cell - derived motor neurons.

At a time when scientists struggled to maintain viable cell cultures, cells derived from her biopsy proved uniquely able to survive and proliferate in culture — in fact, early HeLa cultures doubled every 24 hours!

Bulk - cultured PC3 or PC3 - GFP cells, or clone - derived cultures were plated on glass coverslips and used in immunofluorescence staining for CD44, α2β1 integrin, and β - catenin using monoclonal antibodies and protocols described previously (27 — 29).

Cells were cultured in human foetal RPE medium [30] where, within 13 days, they formed a pigmented monolayer with characteristic RPE cobblestone appearance similar to that observed in human post-mortem RPE sheets and HESC - derived RPE cells [9](Fig.Cells were cultured in human foetal RPE medium [30] where, within 13 days, they formed a pigmented monolayer with characteristic RPE cobblestone appearance similar to that observed in human post-mortem RPE sheets and HESC - derived RPE cells [9](Fig.cells [9](Fig. 1B).

In vitro assays of the osteoblast cultures derived from the MT1 - / - and MT2 - / - mice showed a cell intrinsic defect in the proliferation, differentiation and mineralization abilities of MT2 - / - osteoblasts compared to WT counterparts and the mutant cells did not respond to melatonin additioIn vitro assays of the osteoblast cultures derived from the MT1 - / - and MT2 - / - mice showed a cell intrinsic defect in the proliferation, differentiation and mineralization abilities of MT2 - / - osteoblasts compared to WT counterparts and the mutant cells did not respond to melatonin additioin the proliferation, differentiation and mineralization abilities of MT2 - / - osteoblasts compared to WT counterparts and the mutant cells did not respond to melatonin addition.

For EBI2 transduction of BM - derived cells, BM cells were harvested 4 days after 5 - flurouracil (Sigma) injection and cultured in the presence of recombinant IL - 3, IL - 6, and mouse stem cell factor (SCF)(100 ng / ml; Peprotech; Rocky Hill, NJ).

Ohara, H. Ichikawa, S. Matsumoto, H. Akiyama, M. Fujimoto, N. Kobayashi, T. and Tajima, S. (2010) Collagen — derived dipeptide, proline - hydroxyproline, stimulates cell proliferation and hyaluronic acid synthesis in cultured human dermal fibroblasts.