For mouse ILC3s, splenocytes from Rag1 - / - mice were sorted (Lin -(CD3, CD45R, CD11c, GR - 1, NK1.1) c - kit + Thy1.2 +) and cultured as
described for human cells using recombinant mouse cytokines.
Not exact matches
One patient,
described in a paper in The Lancet, survived
for 2 years after receiving the artificial trachea, which incorporated
human stem
cells.
A physician and
cell biologist who won a 1972 Nobel Prize
for his work
describing the structure of antibodies, Edelman is now obsessed with the enigma of
human consciousness — except that he does not see it as an enigma.
In their findings, reported in Nature Physics, the researchers
describe a method they developed
for growing tiny «brains on chips» from
human cells that enabled them to track the physical and biological mechanisms underlying the wrinkling process.
The laboratory process,
described in the journal Scientific Reports, entails genetically modifying a line of
human embryonic stem
cells to become fluorescent upon their differentiation to retinal ganglion
cells, and then using that
cell line
for development of new differentiation methods and characterization of the resulting
cells.
Coffin
described how lab workers there had transplanted
human prostate tumor
cells into an immune - deficient lab mouse, a common procedure
for procuring a colony of
cells, or a
human cell line,
for further study.
He is part of the team that cloned the gene
for the
human T
cell receptor in 1984, which he
describes as «the Holy Grail of immunology.»
Writing in the journal PLoS Pathogens, the team led by Professor Sachdev Sidhu, of the Donnelly Centre
for Cellular and Biomolecular Research and Department of Molecular Genetics,
describe how they turned ubiquitin, a staple protein in every
cell, into a drug capable of thwarting MERS in cultured
human cells.
As
described in the journal Neurology, Drexel University and Boston University recently received funding from the U.S. Department of Defense to create a Gulf War Illness
human stem
cell repository, to be shared with researchers across the country
for a deeper understanding of this disease.
In this study we
describe the design and pre-clinical evaluation of a CXCR4 - specific ZFN pair (X4 - ZFNs) that specifically and efficiently disrupts cxcr4, rendering
human CD4 + T
cells permanently resistant to HIV - 1 strains that require CXCR4
for infection.
His recent published work
describes the rescued visual function in animals using retinal pigment epithelial
cells derived from
human embryonic stem
cells and a method
for deriving stem
cells using a single -
cell approach that does not harm embryos.
While updating my database of
human clinical trials using expanded
human umbilical cord mesenchymal
cells I came across a new article (April, 2016)
describing a 36 - month safety in subjects given intravenous
cells for in a type 2 diabetes study.
Here we
describe a method
for fabricating a primary
human Small Intestine - on - a-Chip (Intestine Chip) containing epithelial
cells isolated from healthy regions of intestinal biopsies.
Recent papers published by groups from UC Berkeley and Stanford University have
described methods
for the genetic manipulation of primary
human hematopoietic stem and progenitor
cells (HSPCs) that require them to be maintained in vitro
for anywhere from 24 hours to more than a week.
The appearances under phase - contrast microscope of
human satellite
cell cultures during proliferation and differentiation are shown in Figs. 1a and b. Cell cultures were allowed to differentiate for 4 days before cell cultures were exposed to the different insulin concentrations for 4 days as described in research design and meth
cell cultures during proliferation and differentiation are shown in Figs. 1a and b.
Cell cultures were allowed to differentiate for 4 days before cell cultures were exposed to the different insulin concentrations for 4 days as described in research design and meth
Cell cultures were allowed to differentiate
for 4 days before
cell cultures were exposed to the different insulin concentrations for 4 days as described in research design and meth
cell cultures were exposed to the different insulin concentrations
for 4 days as
described in research design and methods.
Human satellite
cell cultures were precultured
for 4 days at different insulin conditions, and the glucose transport activity was determined under basal and after acute insulin stimulation as
described under research design and methods.
Human satellite
cell cultures were precultured
for 4 days to different insulin concentrations, and the content of intracellular glucose and G6P was determined in the basal and insulin - stimulated state and glycogen was determined in the basal state in cultures as
described in research design and methods.
Here we tested whether
human NSCs could be reprogrammed into iPS
cells utilizing a similar strategy as
described above since they represent a more clinically relevant source of
cells for basic studies and modeling
human disease.
Normal
human lung fibroblasts (NHLF)
cells grown in 384 - well Optilux plates were treated with candidate compounds and incubated
for 18 hours prior to fixation and staining with Hoescht dye and anti-HMOX1 antibody as
described in Materials and Methods.
The reprogramming strategy
described here revealed a potential transcriptional signature
for human iPSCs yet retaining the gene expression of donor
cells in
human reprogrammed
cells free of viral and transgene interference.
Now, a new study from the lab of Faris Farassati (Kansas City Veteran Affairs Medical Center, MO, USA)
describes the first mutated version of herpes simplex virus ‐ 1 (HSV ‐ 1) that transcriptionally targets CD133 +
cells, a marker
for CSCs in many
human cancers.
Here, authors from Corning Life Sciences
describe dissolvable microcarriers as a scalable solution
for large - scale expansion and harvest of functional
human mesenchymal stem
cells by enabling simplified downstream processing and high - yield
cell recovery.
Here we
describe the idea, its potential utility, early proofs - of - concept, and some design considerations
for the
Human Cell Atlas, including a commitment to open data, code, and community.
Year 6 Science Assessments and Tracking Objectives covered:
Describe how living things are classified into broad groups according to common observable characteristics and based on similarities and differences, including micro-organisms, plants and animals Give reasons for classifying plants and animals based on specific characteristics Identify and name the main parts of the human circulatory system, and describe the functions of the heart, blood vessels and blood Recognise the impact of diet, exercise, drugs and lifestyle on the way their bodies function Describe the ways in which nutrients and water are transported within animals, including humans Recognise that living things have changed over time and that fossils provide information about living things that inhabited the Earth millions of years ago Recognise that living things produce offspring of the same kind, but normally offspring vary and are not identical to their parents Identify how animals and plants are adapted to suit their environment in different ways and that adaptation may lead to evolution Recognise that light appears to travel in straight lines Use the idea that light travels in straight lines to explain that objects are seen because they give out or reflect light into the eye Explain that we see things because light travels from light sources to our eyes or from light sources to objects and then to our eyes Use the idea that light travels in straight lines to explain why shadows have the same shape as the objects that cast them Associate the brightness of a lamp or the volume of a buzzer with the number and voltage of cells used in the circuit Compare and give reasons for variations in how components function, including the brightness of bulbs, the loudness of buzzers and the on / off position of switches Use recognised symbols when representing a simple circuit in a
Describe how living things are classified into broad groups according to common observable characteristics and based on similarities and differences, including micro-organisms, plants and animals Give reasons
for classifying plants and animals based on specific characteristics Identify and name the main parts of the
human circulatory system, and
describe the functions of the heart, blood vessels and blood Recognise the impact of diet, exercise, drugs and lifestyle on the way their bodies function Describe the ways in which nutrients and water are transported within animals, including humans Recognise that living things have changed over time and that fossils provide information about living things that inhabited the Earth millions of years ago Recognise that living things produce offspring of the same kind, but normally offspring vary and are not identical to their parents Identify how animals and plants are adapted to suit their environment in different ways and that adaptation may lead to evolution Recognise that light appears to travel in straight lines Use the idea that light travels in straight lines to explain that objects are seen because they give out or reflect light into the eye Explain that we see things because light travels from light sources to our eyes or from light sources to objects and then to our eyes Use the idea that light travels in straight lines to explain why shadows have the same shape as the objects that cast them Associate the brightness of a lamp or the volume of a buzzer with the number and voltage of cells used in the circuit Compare and give reasons for variations in how components function, including the brightness of bulbs, the loudness of buzzers and the on / off position of switches Use recognised symbols when representing a simple circuit in a
describe the functions of the heart, blood vessels and blood Recognise the impact of diet, exercise, drugs and lifestyle on the way their bodies function
Describe the ways in which nutrients and water are transported within animals, including humans Recognise that living things have changed over time and that fossils provide information about living things that inhabited the Earth millions of years ago Recognise that living things produce offspring of the same kind, but normally offspring vary and are not identical to their parents Identify how animals and plants are adapted to suit their environment in different ways and that adaptation may lead to evolution Recognise that light appears to travel in straight lines Use the idea that light travels in straight lines to explain that objects are seen because they give out or reflect light into the eye Explain that we see things because light travels from light sources to our eyes or from light sources to objects and then to our eyes Use the idea that light travels in straight lines to explain why shadows have the same shape as the objects that cast them Associate the brightness of a lamp or the volume of a buzzer with the number and voltage of cells used in the circuit Compare and give reasons for variations in how components function, including the brightness of bulbs, the loudness of buzzers and the on / off position of switches Use recognised symbols when representing a simple circuit in a
Describe the ways in which nutrients and water are transported within animals, including
humans Recognise that living things have changed over time and that fossils provide information about living things that inhabited the Earth millions of years ago Recognise that living things produce offspring of the same kind, but normally offspring vary and are not identical to their parents Identify how animals and plants are adapted to suit their environment in different ways and that adaptation may lead to evolution Recognise that light appears to travel in straight lines Use the idea that light travels in straight lines to explain that objects are seen because they give out or reflect light into the eye Explain that we see things because light travels from light sources to our eyes or from light sources to objects and then to our eyes Use the idea that light travels in straight lines to explain why shadows have the same shape as the objects that cast them Associate the brightness of a lamp or the volume of a buzzer with the number and voltage of
cells used in the circuit Compare and give reasons
for variations in how components function, including the brightness of bulbs, the loudness of buzzers and the on / off position of switches Use recognised symbols when representing a simple circuit in a diagram
At TechDay, Audi spent time
describing the issues and its approaches
for constituent elements of different electric drive systems including electric motors and power electronics; battery pack; charging; thermal management; energy recuperation; electromechanical brakes; torque distribution; safety; lightweighting; and the use of a
cell phone in the
human machine interface (HMI).