Sentences with phrase «detect alkaline phosphatases»

Not exact matches

DIG - labeled riboprobes were detected immunocytochemically with Anti-DIG Peroxidase (1 ∶ 500; Roche), TSA Biotin System (PerkinElmer, Salem, MA), Alkaline Phosphatase Streptavidin (1 ∶ 1000; Vector Laboratories, Burlingame, CA) and NBT / BCIP stock solution (Roche) according to the manufacturer's protocol.

Alkaline phosphatase activity was detected in live cells using the Vector Red Alkaline Phosphatase substrate kit (Vector Labphosphatase activity was detected in live cells using the Vector Red Alkaline Phosphatase substrate kit (Vector LabPhosphatase substrate kit (Vector Laboratories).

Following three washes in PBS - T, plates were incubated with pooled sera from malaria - exposed Malawian adults or malaria - naïve UK individuals at a 1:1,000 dilution in PBS - T, 2 % BSA followed by an alkaline phosphatase - conjugated anti-human IgG secondary antibody (Sigma) and detected as above.