We used the Virochip, a microarray designed to
detect all viruses, to identify a new species of
adenovirus (TMAdV, or titi monkey
adenovirus) that caused a deadly outbreak in a colony of New World titi monkeys at the California National Primate Research Center (CNPRC), and also infected a
human researcher.
Adenovirus PCR was performed on a TMAdV - positive clinical sample, a TMAdV culture, and a human adenovirus B culture (as a positive control) using an additional 5 pairs of primers, according to previously published protocols [26], [27], [28] Three of the 5 primer pairs, designed to detect human respiratory adenoviruses B, C, and E, failed to amplify T
Adenovirus PCR was performed on a TMAdV - positive clinical sample, a TMAdV culture, and a
human adenovirus B culture (as a positive control) using an additional 5 pairs of primers, according to previously published protocols [26], [27], [28] Three of the 5 primer pairs, designed to detect human respiratory adenoviruses B, C, and E, failed to amplify T
adenovirus B culture (as a positive control) using an additional 5 pairs of primers, according to previously published protocols [26], [27], [28] Three of the 5 primer pairs, designed to
detect human respiratory
adenoviruses B, C, and E, failed to amplify TMAdV [27].
Although sequencing of PCR amplicons for
human adenoviruses is not performed routinely in diagnostic virology, TMAdV would presumably have been
detected previously in large - scale studies of hexon sequencing of Ad field isolates if it were circulating in the community [46], [47].
Respiratory viral testing failed to
detect evidence of respiratory syncytial virus,
adenovirus, influenza virus A and B,
human metapneumovirus, and parainfluenza virus types 1, 2, and 3.