Sentences with phrase «determine expression levels»

To determine expression levels, in traditional sequencing methods a mix of millions of cells is used.

Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts

Not exact matches

Such modifications, including methylation, acetylation, ubiquitination, and phosphorylation, often result in the alteration of gene expression levels that determine cell fate.

Using quantitative RT - PCR we determined the relative expression levels of the macrophage expressed gene Cd68 in human abdominal subcutaneous adipose tissue.

After a planarian is wounded, the type of injury determines the level fst expression ---- the more extreme the loss of tissue, the higher the level of fst expressed.

mRNA expression from the targeted allele could be detected in embryonic stem cells by RT - PCR (Figure 1D), although expression was reduced approximately 5-fold relative to wt mRNA transcript levels as determined by Northern blotting of RNA from the testis of heterozygous animals (Figure 1E).

They determined that altering the expression level of LIGHT (a specific type of molecule that binds to a receptor site of another cell) and also lymphotoxin (LT) on T cells significantly impacted the cholesterol and triglyceride levels.

Gene - level expression values were represented by fragments per kilobase exon per million reads mapped (FPKM) and a consensus FPKM was determined for each gene based on its representation across biological replicates.

Results from studies with human cells showed highly reproducible expression levels, comparable with those determined by high - throughput RNA sequencing (RNA - seq).

These studies are unable to determine a causal relationship due to factors such as gene regulation — a genetic variant may instead contribute to altered expression levels of true causal genes, which remain undetected by a GWAS.

But transcriptome studies also have significant limitations, such an inability to determine reverse causality — whether gene expression levels are altered by disease, or whether disease arises due to altered gene expression.

Genomes that have been sequenced as part of a GWAS can be run through PrediXcan to generate a gene expression level profile, which is then analyzed to determine the association between gene expression levels and the disease states or the trait of interest being studied.

To quantitatively determine differences between the expression levels of these genes in hNSCs and hESCs, we utilized real time RT - PCR.

Dr Gilchrist said: «This study suggests that we may improve significantly on the widely used analysis methods for determining gene expression levels from high throughput sequence data: absolute quantitation offers a much sounder basis for determining changes in gene expression level, a measure widely used to determine the consequence of genetic, chemical or physical disturbances in living systems.»

The mechanism by which ws1 regulates sex specific wing growth has yet to be determined, although differences in dsx expression level in developing male wings may indicate a role for this sex determination locus.

Additionally, two inflammation markers were determined, namely, the expression levels of cyclooxygenase 2 and the levels of secreted monocyte chemoattractant protein - 1.

Additionally, using RNA - Seq, we determined changes in gene expression levels between the wild - type (WT) and DAXX knock - down (K / D) PC3 cells.

Quadrants were determined by naïve CD4 T cells expression levels.

How does regulation of gene expression determine inner ear function on a genetic and epigenetic level?

After calculating the mean and standard deviation of our datasets, we determined proteins with expression levels below the mean minus the standard deviation as not expressed to discretise the gene expression to an on / off value (see Methods for details).

Our research and product development teams continue to conduct studies investigating new compounds, as well as determining how our current products affect our companions at the most basic cellular level, including gene expression.

73 Moreover, as regards those resources, the expression «recourse to the social assistance system» in Article 7 (1)(c) of Directive 2003/86 does not allow a Member State to refuse family reunification to a sponsor who proves that he has stable and regular resources which are sufficient to maintain himself and the members of his family, but who, given the level of his resources, will nevertheless be entitled to claim special assistance in order to meet exceptional, individually determined, essential living costs or income support measures (see Chakroun, paragraph 52).

This study sought to determine whether use of cannabis in adolescence increases the risk for psychotic outcomes by affecting the incidence and persistence of subclinical expression of psychosis in the general population (that is, expression of psychosis below the level required for a clinical diagnosis).