Sentences with phrase «determine gene expression»

The Core provides equipment and services to determine gene expression patterns in mouse and human tissues.

Hughes's winning research proposed using microarray techniques to compare the genomes of a variety of vertebrate animals to test for common regulatory elements that determine gene expression.

Chromatin is a complex formed by DNA and histones — proteins tightly bound to DNA — packaging it into chromosomes and determining gene expression, a process known as epigenetics.

«These findings raise the possibility that by determining the gene expression profile of a patient's tumor, physicians may be able to identify aggressive disease at the outset of diagnosis and start treatment earlier,» said Sungyong You, PhD, an instructor in the Cedars - Sinai Department of Surgery and the first author of the study.

Dr Gilchrist said: «This study suggests that we may improve significantly on the widely used analysis methods for determining gene expression levels from high throughput sequence data: absolute quantitation offers a much sounder basis for determining changes in gene expression level, a measure widely used to determine the consequence of genetic, chemical or physical disturbances in living systems.»

Not exact matches

The net effect of both the assumptions and the conclusion is that some boys are born into situations in which the combination of gene expression and social context heavily determine what kind of person they will be.

The environment determines the expression of our genes, and a nurturing environment can suppress things like depression.»

Your baby's abilities are just as unique as the way she looks yet somehow it is easier to accept the expression of eye - color genes than those that determine when your baby will hit a particular milestone.

A single miRNA can ramp down expression of multiple genes — but the number of genes affected by known miRNAs hasn't been determined.

A team of scientists led by the University of Birmingham has shown how a common mRNA modification, N6 - methyladenosine (m6A), regulates gene expression to determine the sex of fruit flies.

It was therefore essential to determine whether a causal link exists between methylation and gene expression, rather than relying on a simple correlation.

Rho, found in many bacteria, acts as a protein regulator of bacterial gene expression, determining which genes get turned on or off.

«We used the Allen Human Brain Atlas data to quantify how consistent the patterns of expression for various genes are across human brains, and to determine the importance of the most consistent and reproducible genes for brain function.»

The two samples are each labelled with a different colour fluor so that the relative expression of each gene can be determined for each sample.

Traditionally, gene function was determined one gene at a time, but today's microarray technology enables scientists to monitor the expression of tens of thousands of genes on a single glass slide.

The mGlu2 decrease, they determined, resulted from an epigenetic change, which affects the expression of genes, in this case the gene that codes for mGlu2.

It plays an important role in how cells sense their neighbors and, by controlling gene expression, determines which cells should develop into different types and how much they should grow - like a master controller.»

Indeed, this is true in some cases such as eye color, a characteristic that is determined by gene expression early in development.

First, samples of leaves from these plants are collected for in vitro cultures to isolate the fungi; then the DNA and RNA of fungi are extracted to sequence them and, through bioinformatic analysis, the researcher can determine the expression, the presence or absence of genes in the genomes of a species against each other.

Other researchers used new techniques «to compare patterns of gene expression, to determine what was stressing the bees,» said Berenbaum.

The researchers discovered that the Activin pathway controls the ability of cocaine to induce this change in the neurons and determined that the Activin receptor may control this response to cocaine by regulating the expression of a number of genes.

Rao's team used various approaches, including assays of telomerase activity, measurement of cell growth, and gene expression profiling of TSY - 1 treated cells, to determine how it acts.

The aim now is to identify the molecules that regulate expression of this gene upstream, and those that interact with the enzyme, to understand their modes of action and determine how normal MOCOS expression could be restored..

Using the CAGE (Cap Analysis of Gene Expression) high - throughput method, the scientists determined the starting points of transcription of many thousands of genes in various phases of embryonic development of zebrafish.

The expression of specific genes in a particular root cell determine its fate — the zone in which it will function, Subramanian explained, so he is identifying which micro-RNAs direct gene expression to achieve this differentiation.

To determine whether shifts in the balance of anterior and posterior field occurred during fin - to - limb evolution, Onimaru, postdoctoral researcher currently at Sharpe's lab (CRG), and his colleagues carefully compared the expression, function and regulation of genes involved in anterior - posterior patterning in pectoral fins of catsharks, with those of mice.

Such modifications, including methylation, acetylation, ubiquitination, and phosphorylation, often result in the alteration of gene expression levels that determine cell fate.

The expression rates of three macrophage - specific genes (Emr1, Cd68, and Csf1) that correlated with body mass in our microarray studies, an adipocyte - specific gene (Acrp30), and proinflammatory genes (Tnfa, Nos2, Il6) were determined by quantitative RT - PCR.

Following isolation, RNA was extracted from each population, and the expression of three proinflammatory genes (Tnfa, Nos2, and Il6) was determined by quantitative RT - PCR.

In the current study, prediction analysis of gene expression data was implemented in order to identify the genes that are most useful to determine the state of cyclic changes in locomotor activity.

Using quantitative RT - PCR we determined the relative expression levels of the macrophage expressed gene Cd68 in human abdominal subcutaneous adipose tissue.

(C) Gene expression was greater for Ppard, Pgc - 1α, and citrate synthase in muscles from IL - 15Rα — KO mice, as determined by qPCR.

Cultured mouse, monkey, and human pluripotent cells can be driven down a retinal lineage as determined by the expression of marker genes, including the EFTFs [2]--[4].

Gene Set Enrichment Analysis (GSEA) is a microarray data - mining technique used to determine whether there is coordinated differential expression or «enrichment» in a set of functionally related genes when comparing control and experimental samples [35].

Additional studies are attempting to determine if the transgene expression of various anti-apoptotic genes can protect T cells from tumor - induced apoptosis.

To determine whether or not increased DNA gain or loss likely had an evolutionary impact we compared human and mouse gene expression divergence.

Werner, S. L., Barken, D. & Hoffmann, A. Stimulus specificity of gene expression programs determined by temporal control of IKK activity.

Gene - level expression values were represented by fragments per kilobase exon per million reads mapped (FPKM) and a consensus FPKM was determined for each gene based on its representation across biological replicaGene - level expression values were represented by fragments per kilobase exon per million reads mapped (FPKM) and a consensus FPKM was determined for each gene based on its representation across biological replicagene based on its representation across biological replicates.

These studies are unable to determine a causal relationship due to factors such as gene regulation — a genetic variant may instead contribute to altered expression levels of true causal genes, which remain undetected by a GWAS.

PHOTO: Harbour LaboratoryUsing very small amounts of tumor tissue collected by a needle biopsy, doctors can conduct gene expression profile testing to determine the likelihood that an ocular melanoma tumor will spread beyond the eye.

To understand why skin cells in diverse parts of the body have different characteristics - how cells know their «positional identities» - a fact that guides the diagnosis and treatment of many skin diseases, Dr. Chang and his colleagues are seeking to define in molecular terms how the expression of different genes in stromal cells determines their ability to affect the development of skin cells.

As calculations are based on DNA sequence data and not physical measurements, it can tease apart the genetically determined component of gene expression from the effects of the trait itself (avoiding reverse causality) and other factors such as environment.

«Without computer analysis of sequencing results, we would not have been able to determine relationships between DNA methylation, how accessible regions of the genome were, global gene expression, or the emergence of cancer cells,» says González - Avalos, a graduate student in UCSD's Bioinformatics Graduate Program.

But transcriptome studies also have significant limitations, such an inability to determine reverse causality — whether gene expression levels are altered by disease, or whether disease arises due to altered gene expression.

Genomes that have been sequenced as part of a GWAS can be run through PrediXcan to generate a gene expression level profile, which is then analyzed to determine the association between gene expression levels and the disease states or the trait of interest being studied.

By determining how changes in gene expression affect the fate of glial cells in mice, we hope to understand the key factors that govern neural cell regeneration in the human brain.

To quantitatively determine differences between the expression levels of these genes in hNSCs and hESCs, we utilized real time RT - PCR.

The resulting datasets of regulatory genomic sequence will provide a valuable resource in determining how non-coding DNA sequence variation may impact an individual's capacity to drive appropriate gene expression.

In a paper published in Nature Genetics, an interdisciplinary research team of scientists from the Centre for Genomic Regulation (CRG)-- including a Centro Nacional de Análisis Genómico (CNAG - CRG) group — in Barcelona, Spain, shows that the three - dimensional organisation of the genome plays a key role in gene expression and consequently in determining cell fate.

She will determine which cells harbor the most latent and productive HIV and examine the cells» total gene expression to determine how the gut microenvironment contributes to their long - term survival.