Stepwise synthesis of a nucleic acid to
determine nucleotide sequences; inspired the DNA sequencing technique by F.Sanger (Sanger, Nobel Prize Lecture 2004).
Not exact matches
The specific
sequence of
nucleotides determines the various kinds of life that appear in evolution.
In the words of chemist - turned - philosopher Michael Polanyi, the
sequence of
nucleotides is «extraneous to» the physical and chemical properties within the molecule» which is to say, the
sequence is not
determined by inherent physical - chemical forces.
They use one of several proprietary methods to
determine the
sequence of
nucleotide «letters» (A, C, G and T) that encode genetic information.
Which genes are activated and which are turned off is in part
determined by a second tier of information which is superimposed on the
nucleotide sequences that provide the blueprints for protein synthesis.
That sort of resolution should be good enough to
determine the
sequence of all the
nucleotide bases in the human genetic code.
We have
determined additional full - length
sequences from two clusters of imported EBOV infections into Mali, and we show that the
nucleotide substitution rate (1.3 × 10 — 3 substitutions per site per year) is consistent with rates observed in Central African outbreaks.
A family of neuropeptide genes which includes the gene encoding ELH along with two additional genes encoding the A and B peptides thought to initiate the egg - laying process has been isolated and their
nucleotide sequence has been
determined.
This effort involves first generating lots of tiny, overlapping bits of DNA that must be assembled in the right order after their
nucleotide sequences are
determined.
DNA
sequencing is a process for
determining the precise order of letters (
nucleotides — A, C, G, and T) strung together in a DNA molecule.
To this end, we
determined the susceptibility of 38 NAPCR1 isolates to ten antibiotics from seven classes using E-tests or macrodilution tests and examined 31 NAPCR1 whole - genome
sequences to identify single
nucleotide polymorphisms and genes that could explain the observed resistance phenotypes.
Maternal haplotypes were
determined by
sequencing 655 bp of the mitochondrial D - loop (
nucleotide 15453 — 16107) in 48 English Bulldogs as described [18].