Sentences with word «diaminobenzidine»

Following biotinylated secondary antibody incubation and avidin — biotin complex treatment, immunopositive signals were visualized by diaminobenzidine staining.

Immunostaining was detected with diaminobenzidine (Sigma - Aldrich).

Stainings were developed with 0.06 % 3,3 ′ - diaminobenzidine (Sigma - Aldrich, USA) and 0.01 % hydrogen peroxide (Sigma - Aldrich, USA) in PBS and then mounted on subbed slides.

Detection of specific staining was performed with an anti-mouse HRP - conjugated secondary antibody and diaminobenzidine (DAB) substrate - chromogen (EXPOSE Mouse and Rabbit HRP / DAB Detection Kit, Abcam, Cambridge, MA) according to manufacturer's protocol.