Sentences with phrase «diaminobenzidine staining»
Following biotinylated secondary antibody incubation and avidin — biotin complex treatment, immunopositive signals were visualized by diaminobenzidine staining.
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Stainings were developed with 0.06 % 3,3 ′ - diaminobenzidine (Sigma - Aldrich, USA) and 0.01 % hydrogen peroxide (Sigma - Aldrich, USA) in PBS and then mounted on subbed slides.
Antibodies are labeled with DAB (3,3» - diaminobenzidine) and the resulting brown staining indicates where an antibody has bound to its corresponding antigen.