- Upon completion of imaging, the brainstem /
diencephalon specimen was serially sectioned (axially through the brainstem, coronally through the diencephalon) using a cryomycrotome.
The atlas was generated using several sources of data: 1) histologic data from a dissected ex vivo human brainstem /
diencephalon specimen; 2) correlative diffusion data from the same specimen (scanned prior to serial sectioning and staining); and 3) cross-reference to the Paxinos human brainstem atlas (Paxinos G, Xu - Feng H, Sengul G, Watson C. Organization of Brainstem Nuclei, in The Human Nervous System, 3rd ed.