Sentences with phrase «different antibody conjugates»

Once optimal detector gains were determined, an extensive brightness index of dyes was established on this instrument using sequential single staining of mouse splenocyte with 43 different antibody conjugates against mouse CD8a (53 - 5.8).

Not exact matches

For double labeling, the cells were incubated with primary antibodies against TuJ1 and L1 or GFAP, then biotinylated anti-mouse or rabbit (rat) IgG (Vector, Burlingame, CA, USA), and finally with avidin - rhodamine (Vector) and corresponding FITC - conjugated secondary antibodies (from different host species, Chemicon and Molecular Probes, Invitrogen).

Antibody drug conjugates (ADCs) represent a challenge for bioanalytical scientists, due to their inherent heterogeneity (i.e., different Drug - to - Antibody Ratios), high molecular weight, presence in complex biological matrices and changes in ADC composition in biological samples.