Sentences with phrase «different cell assays»

We will extend our HTS tests using different cell assays and extended chemical components libraries.

Not exact matches

Professor Dianna Bowles, a plant biochemist and founding Director of CNAP, led the research with Professor Paul Kaye, the Director of CII, who developed the robust assay system involving human cells to assess the impacts of the different structures.

By establishing an assay system where free virus infection is restricted and infection can only occur through cell - cell transmission, the researchers could then test whether a large selection of Nabs could prevent cell - to - cell transmission of different HIV strains.

Once developed, the assays will be used to analyse in vitro cytokine and chemokine induction, e.g. to compare different versions of a vaccine, or the same vaccine produced in different host cells, or different batches of a vaccine to demonstrate batch - to - batch reproducibility.

This will include testing the mechanism of action of different adjuvant and antigen formulations by measuring B - and T - cell responses and by using various functional assays (ELISA, FACS, cytokine profiles), transcriptomics and / or proteomics.

«By getting me involved in the project, not only could we do these biological, cell - based functional assays that Roy's lab carries out, but we could also purify the integrins or parts of (them) and do both structural and biophysical characterization of interactions — how the different subunits stick together,» he said.

I completed my PhD as a cell biology major in an engineering lab, where I established a new assay to answer questions very different from what the main part of the lab was working on.

After 24 h of irradiation, amount of cell death in different groups was measured using TUNEL assay (EMD Millipore, Cat.

This allows for a one - step PCR reaction and pooling of amplicons from several different samples and targets (generated with other LymphoTrack Assays for the Illumina ® MiSeq ® instrument, sold separately), onto one MiSeq ® flow cell allowing for up to 24 samples per target to be analyzed in parallel in a single run.

(B and C) Western blot and immunofluoresence assay of Jurkat cells transfected with wild - type (WT), KMT2D mutants (V5486M)(B) and EP300 mutants (H1377R)(C) upon treatment with different HDAC inhibitors.

The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.

Different in vitro assays were carried out in order to test the specific effects of kahweol treatment on several key steps of the angiogenic process in both endothelial and tumor cells.

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

Data are given as percentage, taking the normalized levels of COX - 2 in control cells as 100 %, and they are means ± S.D. of three different assays.

(A) EC50 values from MTT assay using silver nanoparticles with different coatings and HepG2 cells; (B) Analogous with Neuro - 2 cells; (C) Detection of oxidative stress from Ag - NPs (concentration 5 pM) via formation of protein carbonyls incubated with HepG2 cells.

This suggests that our automatic screening assay is specifically measuring the effect amiodarone HCL has on different populations of cells.

Prior research had developed a number of new compounds making use of a novel drug discovery paradigm which begins with natural products extracted from plants; it then entails selecting synthetic derivatives which demonstrate efficacy in multiple assays testing protection against different factors of the nerve cell damage and death which take place in brain injuries and in age - associated neurodegenerative conditions.