Sentences with phrase «different cell lines with»

In the first step, stainings in different cell lines with the same antibody are reviewed and the results are compared with available protein / gene characterization data for subcellular location.

Not exact matches

With a virtual office you can have many different number all connecting back to a single office line or cell phone.

The work, funded by the US National Human Genome Research Institute, aims to create human cell lines with subtly different genomes in order to test ideas about which mutations cause disease and how.

Then, they produced the hemagglutanin with different combinations of these mutations in an experimental cell line (testing the mutations in H7N9 viruses themselves could be dangerous).

Research based on such false cell lines would undermine the understanding of different cancers and possible treatments, and clutter the scientific literature with bogus conclusions.

Even then, with a completely clean lab highly focused on meticulous technique, a new contamination struck in 2013 — something Myers identified when several different cell lines showed up with exactly the same mutation.

Each branch of the tree is lined with cells sensitive to a different chemical or molecular compound.

«Our partners in the US took 1000 Genomes Project cell lines and treated them with different compounds, so we knew which compound had a toxic effect for each cell line,» explains Julio Saez - Rodriguez, former Research Group Leader at EMBL - EBI now at RTWH Aachen University.

In experiments with cancer cell lines, the PIM1 inhibitors killed cells in a MYC - dependent manner, and in two different mouse models — one in which mice were implanted with patient tumors and the other in which a genetic alteration of MYC predisposes the mice to tumor development — the administration of PIM1 inhibitors resulted in significant tumor regression.

«The comparison showed that the Uppsala cell line was genetically identical with the original tumor whereas the U87MG cell line from ATCC had a different, unknown origin.

The study used a well - known line of pancreatic cancer cells (AsPC - 1) in the laboratory and assessed how well this grew when treated with either the chemotherapy drug gemcitabine or different levels of commercially available chokeberry extract alone, and when treated with a combination of gemcitabine and chokeberry extract.

The cell responded to three very different images of the former president: a line drawing of Clinton laughing, a formal painting of him, and a photograph of him mingling with other dignitaries.

They tried hundreds of different recipes; eventually they discovered that if they mixed liver precursor cells (derived from iPS cells) with two other types of standard human cell lines known to be important for embryonic liver development, then the cells would spontaneously form a 4 to 5 - millimeter 3D structure called a liver bud.

The researchers tested the drug on mice with H. pylori infections from several different cell lines, and found that the drug was effective against the H. pylori while maintaining populations of healthy gut bacteria.

The scientists were thus able to transfer the genetic information from the known fluorescent protein eqFP615 into the DT40 chicken B cell line in order to produce protein variants of the new infrared marker Amrose with different spectral properties.

However, efforts to examine the in vitro behavior of tumor cells with endothelial cells from different anatomical regions have been prohibited because, in large part, of the limited number of organ - derived endothelial cell lines available for study.

The discovery of NGF has also promoted an intensive search for other specific growth factors, leading to the isolation and characterization of a number of proteins with the ability to enhance the growth of different cell lines.

Roman numerals represent different microscopic slides and Arabic numerals different cell lines labelled as follows: (I) wt cells (1) labelled with 2 - μm beads, XpdTTD / TTD cells (2) with 0.79 - μm beads, and XpdTTD / KO cells (3) with no beads; (II) wt cells (1) labelled with 0.79 - μm beads and XpdTTD / † XPCS cells (4) with no beads; and (III) wt cells (1) labelled with 0.79 - μm beads and XpdTTD / † XP cells (5) with no beads.

It should be said that stem cell lines are quite different from normal embryonic development and these results should be interpreted with caution.

Teitell used embryonic stem cell lines that made different types of neurons and studied them with array CGH for comparison.

The overall score encompass several factors: reproducibility of the antibody staining in different cell lines (and if signal strength correlates with RNA expression levels); assays for enhanced antibody validation by using antibodies binding to different epitopes on the same target protein (independent antibodies), by knockdown / knockout of the target protein (genetic methods) and by matching of the signal with a GFP - tagged protein (recombinant expression); experimental evidence for location described in literature.

To directly evaluate the relationship between ABL family kinases and metastasis, we analyzed ABL1 and ABL2 protein abundance in MDA - MB - 231 — derived breast cancer cell lines with different organ metastasis tropisms (19).

We use this in vitro model of spontaneous tumorigenesis to generate a library of cell lines with 25 different de novo JAK1 - activating mutations, 6 of which are targeting two residues, Phe958 and Pro960, located in the hinge region at the periphery of the ATP - binding pocket.

Here, we discuss the toxicity and the cellular uptake of different silver nanoparticles functionalized with citrate, three different monosaccharides as well as ethylene glycol on two different cell lines.

Year 6 Science Assessments and Tracking Objectives covered: Describe how living things are classified into broad groups according to common observable characteristics and based on similarities and differences, including micro-organisms, plants and animals Give reasons for classifying plants and animals based on specific characteristics Identify and name the main parts of the human circulatory system, and describe the functions of the heart, blood vessels and blood Recognise the impact of diet, exercise, drugs and lifestyle on the way their bodies function Describe the ways in which nutrients and water are transported within animals, including humans Recognise that living things have changed over time and that fossils provide information about living things that inhabited the Earth millions of years ago Recognise that living things produce offspring of the same kind, but normally offspring vary and are not identical to their parents Identify how animals and plants are adapted to suit their environment in different ways and that adaptation may lead to evolution Recognise that light appears to travel in straight lines Use the idea that light travels in straight lines to explain that objects are seen because they give out or reflect light into the eye Explain that we see things because light travels from light sources to our eyes or from light sources to objects and then to our eyes Use the idea that light travels in straight lines to explain why shadows have the same shape as the objects that cast them Associate the brightness of a lamp or the volume of a buzzer with the number and voltage of cells used in the circuit Compare and give reasons for variations in how components function, including the brightness of bulbs, the loudness of buzzers and the on / off position of switches Use recognised symbols when representing a simple circuit in a diagram