Sentences with phrase «different cell populations»

To confirm the finding, normal bone marrow cells were exposed to either IMGN632 or X-ADC for 72 hours at a concentration highly active against AML cells (100 pM), and the degree of caspase 3 activation, a marker of apoptosis, in different cell populations was assessed by flow cytometry (supplemental Figure 4).

RNA was extracted from different cell populations using Trizol ™ (Invitrogen) and precipitated with isopropanol.

This requires monitoring the different cell populations that make up the growing tumor and adjusting the schedule based on the data.

Percentage and absolute number were determined for different cell populations.

The experimental plan includes a fine - grained analysis of the different cell populations within these precious tissues using cutting - edge single - cell genomics methods.

It is a bulk method that sorts different cell populations by tuning their solubility.

On the other hand, if the same mutation occurs in a different cell population located farther away from the SVZ, it will give rise to other subtypes,» said Chen.

Not exact matches

However, it remains unclear how these different stem cells populations balance proliferation, differentiation and migration during the healing process.

Scientists in the six - site study analyzed the tissue in 41 rheumatoid arthritis patients, separating out different immune cell populations.

Although the new JNCI study did not analyze individual cell phone usage — preferring instead to take a broader look at the population at large — other research has examined subgroups of cell phone users to try to discern different trends among more frequent use.

Now, a team of scientists at the Icahn School of Medicine at Mount Sinai have developed the Just EGFP Death - Inducing T - cell, or JEDI T - cells, which enable the visualization of T - cell antigens, allowing researchers to study T - cell interactions with different cell types, model disease states, and finally determine the functions of otherwise poorly characterized cell populations.

Different clonal memory cell populations had different B cell or macrophage helper compositions that matched effector cell populations generated much earlier in the Different clonal memory cell populations had different B cell or macrophage helper compositions that matched effector cell populations generated much earlier in the different B cell or macrophage helper compositions that matched effector cell populations generated much earlier in the response.

Human epidermal equivalents representing different types of skin could also be grown, depending on the source of the stem cells used, and could thus be tailored to study a range of skin conditions and sensitivities in different populations.»

Next, they used a technique called fluorescence - activated cell sorting to separate out the newly differentiated retinal ganglion cells from a mixture of different cells into a highly purified cell population for study.

Human epidemiology studies in populations in different geographic locations, as well as clinical and molecular studies, show that Merkel cell polyomavirus causes Merkel cell carcinoma.

Researchers in this study used budding yeast, creating populations of cells with more than 10 million different randomised genomes, to investigate how genetic diversity affected resistance.

For Helicobacter pylori, a pathogenic bacterium that colonizes over 40 percent of the world population and is associated with gastric cancer, the team discovered that epigenetic heterogeneity can quickly emerge as a single cell divides, and different subpopulations with distinct methylation patterns have distinct gene expressions patterns.

In this mouse model, mutations in Kras and p53 genes resulted in the formation of individual tumor cell populations that were labeled with different colors.

Stem cell populations reside in areas called niches deep within different types of organs.

The code that a monkey's brain uses to represent faces relies not on groups of nerve cells tuned to specific faces — as has been previously proposed — but on a population of about 200 cells that code for different sets of facial characteristics.

In addition, they also counted the numbers of various types of single - celled organisms called testate amoebae, whose population changes depending on the amount of water in the bog, giving an indication of how wet or dry the climate was during different periods.

Tumors, for example, are known to contain different populations of cells, some of which are quite different to each other in their genetic makeup.

In the different cell types, the loops begin and end at different specific chromosomal locations, so each cell line's genome appears to have a unique population of loops.

«It was very striking that the two cell populations had such different growth potentials.

A single tumor is composed of many different populations of cells.

Their recent study, which appears as the cover article in the May issue of Cancer Research, shows that mathematical models can be used to predict how different tumor cell populations interact with each other and respond to a changing environment.

Nervous system in Nematostella vectensis embryos with different nerve cell populations, where the different neurons (here in green, blue and magenta) evidence asymmetry.

When the environments are made more different, new cell populations are recruited in CA3 but not in the dentate gyrus.

To look at the effects of heat shock on native populations of proteins, Drummond and his colleagues utilized a novel set of techniques that allowed them to simultaneously track almost 1,000 different mature proteins in yeast cells.

From these three cell populations different functional tissue cells arise, such as skin cells, muscle cells, and specific organ cells.

In principle, tissue banks of parthenogenetic cell lines could include enough different immune protein combinations to treat up to half of the U.S. population — men as well as women — Lanza says.

The researchers tested the drug on mice with H. pylori infections from several different cell lines, and found that the drug was effective against the H. pylori while maintaining populations of healthy gut bacteria.

Researchers at the Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I - Stem — Inserm / AFM / UEVE), in collaboration with CNRS and Paris Descartes University, have recently developed a new approach to better control the differentiation of human pluripotent stem cells, and thus produce different populations of motor neurons from these cells in only 14 days.

It describes how a population evolves from different mating types producing same - sized sex cells (or gametes) to mating types producing different - sized gametes, such as where there are distinct males and females.

This phenomenon, which indicates the presence of different mitochondrial DNA types in a cell or an individual, can be found in more than half of the population.

The selection strategy targeted cell populations expressing the inducible endothelial cell adhesion molecules, E-selectin and VCAM - 1, and proved successful in generating microvascular endothelial cell lines from a number of different organs.

This murine model system offers a superior source of immortalized cell populations as many of the difficulties associated with the in vitro transfection process are eliminated (e.g., initial requirement for many cells, different sites of gene integration, multiple copy number; Ref.

Researchers at the Institute for Stem Cell Therapy and Exploration of Monogenic Diseases (I - Stem — Inserm / AFM / UEVE) have recently developed a new approach to produce different populations of motor neurons from these cells in only 14 days.

Other forms of cell - cell interaction, on the other hand, are only effective in mixed population structures; these include, for example, cross-feeding mutualisms in which different cell types depend on close proximity to benefit each other [17 — 19].

With regard to HgT, a molecular analysis of cybrid cells with identical nuclear backgrounds and different mtDNA populations indicated that HgT cybrids had a higher survival rate and were more tolerant of oxidative stress when compared to the more common European haplogroup H [45].

As if that had not been complicated enough already, it has been discovered that even the very same tumor can harbor multiple different stem cell populations in its midst!

«[It] supports this idea that chronic inflammation in children, perhaps through exposure to bacterial endotoxin in the gut, is causing these different immune cell populations to be non-responsive to cytokine stimulation,» Petri said.

A major new insight is the realization that different tumors of the same type may rely on entirely different tumor stem cell populations.

Alterations accomplished using CRISPR, which enables scientists to edit a cell's DNA with unprecedented precision, are different in one crucial respect: The process can result in «gene drive,» a naturally occurring feature of some genes that enables them to spread through a population over generations, even if they do not help survival (and thus reproduction).

He is also interested in understanding the transcriptional regulation of lineage choice between different stem cell populations that occurs in the blastocyst.

The players are the healthy cells, the targeted cancer cells and the resistant cancer cells and the game is played over and over again in the computer simulation as the different populations of cells compete.

We show that subpopulations of human astrocytes, generated by activation of different signaling pathways in the same population of human glial precursor cells, have markedly different effects when transplanted into the injured spinal cord.

When placed under different promoters, Brainbow and Flpbow can be used to label distinct cell populations.

Immune cell subtypes might shift in someone who has an infection or an allergy or an autoimmune disease, for example; or they may vary for different population groups.

Our findings suggest that pluripotency is determined by the capacity of a mixed population of lineage - biased intermediates to commit to different cell fates in specific contexts.