Similarly, if you wanted to study environmental factors that influence sleep, you could examine sleeping patterns of
different species in the wild and attempt to relate them to differences in temperature,
photoperiod, or other factors.
The chemical composition of the microalgae is not an intrinsically constant factor, but varies over a wide range in accordance with the
different cultivation factors such as temperature, light (intensity, wavelength and
photoperiod), pH of the medium, supply of CO2, concentration and type of nutrients, source and concentration of nitrogen, salinity and growth phase.