The team rewrote the Microsoft formal verification program, then fed genetic and chemical data from
the different stem cell cultures into it — with some surprising results.
Not exact matches
Despite a lack of rigorous clinical trials to test safety or efficacy, a ban of the treatment by health authorities, and a lack of peer review by the scientific community, the privately funded Stamina Foundation has been using
cultured mesenchymal
stem cells that have been exposed to putative conditions that favour neuronal differentiation to treat
different diseases in severely or terminally ill patients.
Part of the trouble is the ingredients: Subtle variations in tissue -
culture chemicals and Matrigel, or in
different stem cell lines and how they are grown first in 2 - D
culture, can have a big impact on how the organoids turn out, Novitch says.
Moving forward, he was able to place these taste
stem cells in a
culture dish and prompt them to grow into the
different mature taste
cell types, thus creating a taste bud in a dish — scientifically known as taste organoids.
After experimenting with several
different cocktails, the researchers hit on a mix that transformed 100 percent of
cultured stem cells into those that looked like neurons in under an hour.
In the new study, the researchers
cultured mouse skin - cancer colonies on various 2 - D and 3 - D environments of
different shapes and patterns to see if the tumor shape contributes to activation of cancer
stem cells, and to see where in the tumor the
stem cells appeared.
«Our group pioneered the development of
cell culture technology for harvesting large numbers of
stem cells from human bone marrow and human umbilical cord blood,» Dr. Yeh said, noting that
stem cells from these two sources are abundant and can be guided into
different types of
cells using tissue engineering.
«Our
stem cells also survive outside of mice, in a
culture, so we can also manipulate them in a laboratory,» said Abad, adding that: «The next step is studying if these new
stem cells are capable of efficiently generating
different tissues such as that of the pancreas, liver or kidney.»
The team immersed the
stem cells in four
different types of these
cultures and analysed which genes they expressed in which environment, and to what extent.
Spike and Gray grew the mammary
stem cells in
culture dishes and stained them so that new
stem cells appeared a
different color from differentiated mammary
cells.
Blood
stem cell cultures: Blood
stem cells from colonies (
cell clusters) in vitro consisting of
different blood
cells.
«We wanted to understand how
stem cell transcription factors were interconnected and explain how these
cells behave in
different culture conditions and still maintain the
stem cell state,» said Smith.
In this study, we analyzed three
different cultures of neural
cells carrying trisomy of chromosome 21, which were generated by directed differentiation from induced pluripotent
stem cells (iPS
cells).