In the PROSPECTS project new or improved tools and approaches have been developed to enable absolute protein quantification, study of protein - protein interactions and protein complexes and the quantitative study of protein abundance and turnover rates in
different subcellular localizations at a proteome - wide level.
The ability to label
different subcellular locations with the green fluorescent protein (GFP) has made it possible to visualize intracellular activities in living cells.
Not exact matches
«The cytoskeleton system is comprised of a host of interacting
subcellular structures and proteins, and our technique will enable research on the interactions between these
different targets with unprecedented number of color channels and spatial resolution,» he said.
We demonstrated the technique on 20
different biological processes spanning four orders of magnitude in space and time, including the binding kinetics of single Sox2 transcription factor molecules, 3D superresolution photoactivated localization microscopy of nuclear lamins, dynamic organelle rearrangements and 3D tracking of microtubule plus ends during mitosis, neutrophil motility in a collagen mesh, and
subcellular protein localization and dynamics during embryogenesis in Caenorhabditis elegans and Drosophila melanogaster.
The present study revealed a consistent
subcellular segregation of immunoreactive sites detected by Aβ - specific antibodies and pab27576 within neurons of
different brain areas, at pre - and post-plaque stages of the amyloid pathology.
To further clarify the role of Prx - dependent mitochondrial signaling, we expressed
different forms of dPrx5, which unlike the uniquely mitochondrial dPrx3 is found in multiple
subcellular compartments, including mitochondrion, nucleus and cytosol.
In the first step, stainings in
different cell lines with the same antibody are reviewed and the results are compared with available protein / gene characterization data for
subcellular location.
The core facility is a two - photon in vivo imaging platform developed at the Nonlinear bioimaging laboratory, a technique that allows for non-invasive structural and functional measurements in small animal models at
different scales: from macroscopic imaging of the brain morphology to highly resolved microscopy of neuron populations, single neurons, and even
subcellular structures.
Based on the resource of more than 47,000 antibodies and a panel of > 20
different human cell lines, this facility has a unique position to investigate
subcellular spatial proteomics for human and rodent biology.
Questions remain concerning why neurons that apparently express similar innexins (the
different motor neuron classes) rarely form gap junctions with one another, if gap junctions are subject to constant formation and reformation in the nervous system, and why there appears to be a consistency in
subcellular localization (soma versus process) of certain gap junctions.
The successful candidate will receive extensive training on proteomics - based discovery (secretome proteomics to identify cytokines important in
different children's cancers, protein expression profiling of
subcellular fractions and organelles, and interactome proteomics to identify novel protein — protein interactions) and follow - up of proteomic findings (molecular cell biological characterization of protein functions and signaling).