Sentences with phrase «dissociated cells in culture»

Previously MYC has been shown to promote proliferation and inhibit differentiation in dissociated cells in culture.

Not exact matches

The long - term facilitation of synaptic efficacy that is induced by serotonin in dissociated cell cultures of sensory and motor neurons of Aplysia is accompanied by the growth of new synaptic connections.

The tumor cells were dissociated and the cells were infected with KLF4 lentivirus and cultured in low - attachment plates.

Cells were detached (adherent culture) or dissociated (spherical culture) by trypsin / EDTA and resuspended in PBS for cell counting using the ViaCount assay.

To determine the effect of the conditioned medium on NGF - induced neurite outgrowth, PC12 cells were dissociated and plated on polyornithine - coated tissue culture dishes in the following conditions: 1) HT22 conditioned medium, 2) J147 treated HT22 conditioned medium, 3) DMEM alone plus J147, 4) DMEM plus NGF at 50 nanograms per ml, 5) J147 treated HT22 conditioned medium pre-incubated for one hour with 10 μg / ml anti-NGF and N2 supplement (Invitrogen).

The primary epithelial cells are expanded as 3D organoids, dissociated, and cultured on a porous membrane within a microfluidic device with human intestinal microvascular endothelium cultured in a parallel microchannel under flow and cyclic deformation.

Significant advancements have been made in the bioprocessing of hPS cells including the formulation of new cell culture media, the development of extracellular and acellular matrices that can replace feeder cells, the development of new enzymes for passaging and the discovery of small molecules that significantly enhance the survival and clonogenicity of dissociated cells, allowing single cell cryopreservation.