Sentences with phrase «distilled water twice»

Larvae were washed with distilled water twice, and stored at -80 °C until use.

Not exact matches

To obtain the fat bodies from casein treated or starved larvae, we fed w1118 larvae on normal food until 12 h AL3E and then transferred them into 5 % casein food or 1 % non-nutritive agar, respectively, and incubated for additional 12 h. Larvae were surface - sterilized in 70 % ethanol for a few minutes, rinsed twice in sterilized distilled water, and dissected in a Schneider's Drosophila medium (Biowest) with 10 % FBS (Gibco) and 1 % Penicillin - Streptomycin (Sigma).

Glassware was cleaned by immersion in 30 % glacial acetic acid or 6 M HCl for at least 6 hours, rinsed with distilled water, and autoclaved twice prior to use.

I also drink twice a day one tsp each of chlorella and spirulina in eight ounces of distilled water along with six ounces of herbal tea (green, slippery elm bark etc.), 1 tsp of liquid minerals in four ounces of pure cranberry juice mixed with 4 ounces of distilled water and sixty four ounces of distilled water.

I've started by mixing 2 tablespoons in 16 oz of distilled water and drinking this twice a day (so 4 tablespoons per day total).