Sentences with phrase «donor cell nuclei»

Down arrows point to subretinal donor cell nuclei in panel B, while up arrows point to preserved dendrites of host rod bipolar cells.

Note the location of donor cell nuclei in both the inner retina and subretinal space.

The PPL team used the traditional fusion technique to marry the donor cell nucleus to the egg.

Not exact matches

It is far more likely, however, that the egg - cell cytoplasm with its stripping factor will reprogram all the genetic material including the alterations made in the donor nucleus that were intended to prevent the creation of the zygote.

OAR proponents claim that when the altered donor - cell nucleus with its activated nanog gene is transferred to the enucleated oocyte (egg cell), the presence of nanog will immediately convert the enucleated egg cell to a pluripotent cell, without ever forming a zygote.

The second biological objection is that «the egg - cell cytoplasm» will «reprogram all the genetic material including the alterations made in the donor nucleus that were intended to prevent the creation of the zygote.»

This hypothesis underestimates the opposing power of the egg cell cytoplasm to reprogram the genes in the donor - cell nucleus.

The OAR proposal uses a variation of therapeutic cloning called altered nuclear transfer (ANT) in which the nucleus of a donor cell (a skin cell, for example), containing the 30,000 genes of the genetic code, is altered in such a way that it produces an epigenetic factor, a protein called nanog.

The cell nuclei are removed from both sets of embryonic cells, as shown in the diagram, the donor's nuclei and the remains of the parents» embryo are destroyed and the parents» nuclei are then inserted into the donor or «host» embryo, still containing its healthy mitochondria.

In this process, the nucleus of a donor adult cell is transferred to an egg whose nucleus, and thus its genetic material, has been removed.

Both involve a technique called nuclear transplantation — replacing the nucleus of a donor's egg with the DNA from an adult cell.

OHSU scientists have also demonstrated that SCNT allows replacement of mutated mitochondrial genes with healthy donor egg mitochondria while retaining the patient cell's nucleus.

The scientists removed the DNA - containing nuclei from the eggs and replaced them with DNA from the donor skin cells.

The patent gives California - based Geron Corp. exclusive rights to animal embryos prepared by transferring the nucleus of a quiescent diploid donor cell into a suitable recipient cell up to and including the blastocyst stage.

But the next step — transferring the nucleus — was different; rather than fusing the donor cell with the enucleated egg cell, the team removed the donor nucleus with a very fine needle and injected it into the donor egg.

He tested various methods to quickly and precisely remove the nuclear materials from the egg cell and promote the fusion of the nucleus - donor cell and enucleated egg.

Like Dolly's creators in Scotland, Don Wolf of Portland's Oregon Health Sciences University and his colleagues transferred the nuclei from donor cells to eggs whose own DNA had been removed.

In addition, the sources of the replacement nuclei were the egg donors» own cumulus cells, which encircle developing eggs in the ovary.

The new finding brings a measure of closure to a story that first rocked the science world in February 2004, when Hwang and colleagues at Seoul National University announced they had cloned a female donor's cell by transferring its nucleus into one of her egg cells stripped of its nucleus in a procedure known as somatic cell nuclear transfer (SCNT), and harvested embryonic stem cells from the resulting fusion.

In their initial experiments, Hwang and his colleagues used cells from a single donor to try to create embryonic clones; they transferred nuclei from ovarian cells back into the donors» own oocytes.

Dolly was created using nuclear transfer, a technique in which an intact donor cell is fused with an egg whose nucleus had been removed.

But cell biologist Leonard Guarente of the Massachusetts Institute of Technology says the evidence does suggest that being cloned was able to «restore a youthful state» to the donor cell's nucleus.

Cell nuclei, rod outer segments, and donor - derived retina are blue (DAPI), red (XAP2), and green (Venus YFP), respectively.

You inject the nucleus from Archie's cell into the donor egg.

Animal cloning uses a process known as somatic - cell nuclear transfer, where the nucleus from an adult (donor) cell is transferred into an egg (host) cell lacking a nucleus.

In essence, these studies suggested that a newly fertilized, enucleated egg has the ability to «reprogram» a much older donor nucleus, making the resultant cell behave as though it were at a very early embryonic state.

The next steps in cloning would be to 1) insert a donor nucleus of a new bird into the enucleated egg cell, 2) stimulate embryonic growth, and 3) implant the embryo into a mother bird or egg.