Sentences with phrase «donor fluorophores»
Homogeneous Time - Resolved Fluorescence (HTRF) Utilizing rare - earth lanthanides with long emission half - lives as donor fluorophores, HTRF technology combines standard FRET with the time - resolved measurement (TR) of fluorescence.
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Ligand - mediated receptor assembly was measured by photon transfer from the photon donor to the fluorophore resulting in fluorescence emission.
In their JCB 2003 paper, Sekar and Periasamy defined FRET as «a distance - dependent physical process by which energy is transferred non-radiatively from an excited molecular fluorophore (the donor) to another fluorophore (the acceptor) by means of intermolecular long - range dipole - dipole coupling.»