Sentences with phrase «driven luciferase reporter»

Once they'd found suitable pairs, they constructed three components: GBPa - VP16 (activation domain); GBPb - GAL4 (DNA - binding domain); and a UAS - driven luciferase reporter construct.

To this end, increasing amounts of expression constructs of DDX3 / DQAD, DDX3 / AAA and wild - type DDX3 were cotransfected with p21waf1 / cip1 promoter — driven luciferase reporter.

p21waf1 / cip1 promoter — driven luciferase reporter (p21 - Luc; 0.05 - 0.5 μg) and increasing amount (0.1 - 2 μg) of FLAG - DDX3 expression construct were cotransfected into various cell lines as indicated.

To examine whether DDX3 transactivates the p21waf1 / cip1 promoter activity through Sp1 site - interacting proteins, the Sp1 or Sp3 expression plasmid together with the DDX3 expression construct and the p21waf1 / cip1 promoter — driven luciferase reporter were introduced into HuH - 7 cells.

The liver cells transfected with an HCV internal ribosome entry site (IRES)- driven luciferase reporter that were treated with SBEL1 reduced reporter activity by 50 % compared to control.

The p21waf1 / cip1 promoter — driven luciferase reporters (pGL3 derivatives) containing point mutations in six Sp1 elements, (Mut1) p21 - Luc, (Mut2) p21 - Luc, (Mut3) p21 - Luc, (Mut4) p21 - Luc, and (Mut5 / 6) p21 - Luc and the parental wild - type reporter, (Wt) p21 - Luc, were kindly provided by Dr. D. Kardassis (Department of Basic Sciences, University of Crete Medical School, Heraklion, Crete, Greece; ref.

Next, to examine the underlying mechanism by which DDX3 activates the p21waf1 / cip1 promoter, we mapped the DDX3 responsive region within the p21waf1 / cip1 promoter by serial 5 ′ - deleted promoter - driven luciferase reporters (Fig. 3A and Supplementary Fig.

A, schematic representation of serial p21waf1 / cip1 promoter — driven luciferase reporters used in transactivation assay: p21 - Luc contains the 2.3 - kb full - length of p21waf1 / cip1 promoter (from − 2,326 to +10 nucleotide related transcription start site); its derivatives (− 159 / +8) p21 - Luc, (− 123 / +8) p21 - Luc, (− 84 / +8) p21 - Luc, (− 76 / +8) p21 - Luc, (− 63 / +8) p21 - Luc, and (− 56 / +8) p21 - Luc contain a series of deleted promoters of p21waf1 / cip1.

Not exact matches

The results suggested that the levels of activation induced by DDX3 of the luciferase activity of serial reporter constructs from (− 1,002 / +8) p21 - Luc to (− 123 / +8) p21 - Luc were comparable with the level of activation elicited by the full - length promoter - driven reporter p21 - Luc (5 - to 10-fold; Fig. 3B and Supplementary Fig.