Sentences with phrase «dual luciferase assay»
hco - miR - 9551 was shown to interact with the 3» UTR of this gene by dual luciferase assay.
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Transfected cells were harvested at 48 hours posttransfection and a dual luciferase assay was done according to the instruction of the manufacturer.
Not exact matches
After 24 hours, luciferase activities were measured by using dual - luciferase reporter assay system (Promega).
At 48 hours, cells were washed twice with PBS and lysed in 1 × passive lysis buffer (Dual - Luciferase Reporter Assay, Promega).
Luciferase activities were measured using the Dual - Luciferase Reporter Assay kit (Promega) following the manufacturer's instructions.
The cells were cotransfected with the reporter gene constructs and related expression plasmids as indicated by using SuperFect (Qiagen) followed by transcriptional activity assay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instrucassay using Dual - Luciferase Assay System (Promega) according to the manufacturer's instrucAssay System (Promega) according to the manufacturer's instruction.
The Dual - Luciferase Reporter Assay System (E1910; Promega, Madison, WI) was used to perform dual - reporter assays on NFκB luciferase and Renilla luciferase (internal contrDual - Luciferase Reporter Assay System (E1910; Promega, Madison, WI) was used to perform dual - reporter assays on NFκB luciferase and Renilla luciferase (internalLuciferase Reporter Assay System (E1910; Promega, Madison, WI) was used to perform dual - reporter assays on NFκB luciferase and Renilla luciferase (internal contrdual - reporter assays on NFκB luciferase and Renilla luciferase (internalluciferase and Renilla luciferase (internalluciferase (internal control).
Luciferase activities were measured using the Dual - Luciferase Reporter Assay System (Promega) and normalized to Renilla luciferaseLuciferase activities were measured using the Dual - Luciferase Reporter Assay System (Promega) and normalized to Renilla luciferaseLuciferase Reporter Assay System (Promega) and normalized to Renilla luciferaseluciferase activity.