Sentences with phrase «during cell reprogramming»

Stadhouders R, Vidal E, Serra F, et al., Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming.

The findings reported in Nature Genetics indicate that genome architecture has important informational value for controlling gene expression during cell reprogramming, and is thus required for the specialized functions of a cell.

Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming.

Not exact matches

The MYC protein — which is one of the molecules used to reprogram iPSCs from adult cells — likely plays a role in dictating which sites in the genome are randomly methylated during the reprogramming process, the researchers hypothesized.

Plath's team found that the changes that happen in cells during reprogramming occur in a sequential stage - by - stage manner, and that importantly, the stages were the same across all the different reprogramming systems and different cell types analyzed.

When researchers suppressed the ARF gene in mole - rat cells during the reprogramming process to iPSCs, the cells stopped proliferation with sign of cellular senescence, while the opposite happens with mouse cells.

«As researchers started using these cells more, it became clear that during the process of reprogramming to create stem cells the cell was also rejuvenated in other ways,» says Jerome Mertens, a postdoctoral research fellow and first author of the new paper.

During the first hours after fertilization, the two separate genomes undergo reprogramming events that presumably function to erase the memory of the differentiated cell type and establish a state of totipotency.

After some time during which reprogramming should take place, you start to evaluate the outcome and conduct functional assays, such as patch clamp recordings in case you try to obtain neurons to prove that cells really change their identity.

We have identified a number of key transcription factors that are deregulated during this process, and we are using this information to investigate mechanisms by which differentiation can be reprogrammed in tumour cells.

(2015) Myc and SAGA rewire an alternative splicing network during early somatic cell reprogramming.

His lab has pioneered the generation of clinical grade induced pluripotent stem (iPS) cells using non-viral reprogramming methods such as direct delivery of reprogrammed proteins and novel episomal methods, and has recently identified novel mechanisms underlying metabolic reprogramming during human induced pluripotency.

Compared to MEF cells, which can be maintained for only 5 — 7 days after irradiation, HFF cells as a feeder layer avoid frequent passage and allow the maintenance of established cell - cell interaction during the reprogramming process.

The three - dimensional organization of the genome represents a cell - type specific means to regulate gene transcription; however, we currently lack a detailed understanding of the architectural changes that occur during the generation of induced pluripotent stem cells (iPSCs) due to the inherent inefficiency of the reprogramming process [1].

Previous data in the literature suggested that reprogramming factors should be maintained for up to 12 days during iPSC generation from mouse cells [31], [32].

Reversible Reprogramming of Circulating Memory T Follicular Helper Cell Function during Chronic HIV Infection.

The single - cell perspective has helped to better understand gene regulation and regulatory networks during exit from pluripotency, cell - fate determination as well as molecular mechanisms driving cellular reprogramming of somatic cells to induced pluripotent stage.

Utilising both chondrocytes as a starting material and non-integrating mRNA technology as a reprogramming strategy, the group aimed to remove the risk of epigenetic memory of somatic cell of origin, which can affect future differentiation propensity, and reduces the risk of abnormalities being introduced during reprogramming.