Developmental biologist Gary Wessel and colleagues at Brown University in Providence, Rhode Island, found the answer by studying sea urchin
eggs during fertilization.
This «IVF chip» is a unique tool to observe the cascade of molecular and membrane events
occurring during the fertilization process,» Ravaux said, and under conditions that mimic the natural process.
Previous attempts to obtain information on the dynamical activity of the sperm and egg
membrane during fertilization involved inseminating an egg with multiple sperm, which leads to «non-physiological» situations where many sperm are simultaneously in contact with the plasma membrane of the egg.
In the end, just nine of these eggs passed the final test for maturity — they were able to divide and halve their chromosomes so they were ready to join with
sperm during fertilization, the researchers reported online 30 January in Molecular Human Reproduction.
These resultant haploid cells can fuse with other haploid cells of the opposite sex or mating
type during fertilization to create a new diploid cell, or zygote.
In mammals, germline cells are the sperm and ova (also known as «gametes») which
fuse during fertilization to produce a cell called a zygote, from which the entire mammalian embryo develops.
Only two other examples of plant cell fusion have been observed so far, and over 110 years have passed since the identification of the two cell fusions, which occurred between two sets of
gametes during fertilization.
«Our findings were quite surprising because females typically just select sperm from males of their own
species during fertilization, an action that does not lead to long - term consequences because there is no gene flow between the species,» said Asher Cutter, associate professor of ecology and evolutionary biology at the University of Toronto.
The scientists also tried to find new mutations in the child that neither the father nor the mother carried but that arose during the reproductive process as sperm and egg were created, or as their DNA
meshed during fertilization.
«We believe that TH2A and TH2B in combination enhance reprogramming because they introduce a process that normally operates in the
zygote during fertilization and SCNT, and lead to a form of reprogramming that bears more similarity to oocyte - based reprogramming and SCNT» explains Dr. Ishii.
Soon after the egg and sperm
join during fertilization, the resultant zygote (fertilized egg cell) starts undergoing cell division.
Sperm cells can not divide and have a limited life span, but after fusion with egg
cells during fertilization, a totipotent zygote is formed with the potential to develop into a new organism.
She works in a WSU lab that focuses on evaluating hormonal regulation in the oviduct and
uterus during fertilization, pre-implantation embryo development and embryo transport using genetically engineered animal models.
Researchers have capitalized on the unique properties of a sperm cell to follow cell membrane fusion as it
occurs during fertilization, tracking the full cascade of events for the first time.
Shortly after a sperm penetrates an
egg during fertilization, the sperm's mitochondria are degraded while the egg's mitochondria persist.
The team also noticed that
during fertilization, the many organizing centers disassembled, re-creating the mesh throughout.
During fertilization, the embryo inherits only the centrioles from the sperm, thus having the correct number.