At Berkeley, Yildiz expanded his studies on the movement of
dynein motors and also branched out in a new direction.
Self - organization of
dynein motors generates meiotic nuclear oscillations.
The team at Leeds, working within the world - leading Astbury Centre for Structural Molecular Biology, combined purified microtubules with purified
dynein motors and added the chemical fuel ATP (adenosine triphosphate) to power the motor.
«Each of the two arms of
a dynein motor protein is about 25 nanometres (0.000025 millimetre) long, while the binding sites it attaches to are only 8 nanometres apart.
Not exact matches
To model this molecular
motor, the scientists created a molecular dynamics simulation of a
dynein protein and applied energy at the ATP binding site to approximate the transfer of energy from ATP.
The axoneme's movement is accomplished via rows of
motor proteins called
dyneins that are attached along the microtubules and exert force on them so the microtubules «slide» past each other, which then causes the entire axoneme and sperm tail to bend and move.
They are made up of microtubules and many other proteins, including the
motor protein
dynein.
Researchers at the University of Leeds and in Japan used electron microscopes to capture images of the largest type of
motor protein, called
dynein, during the act of stepping along its molecular track.
For instance, in the
motor neuron connecting the central nervous system to the big toe — which is a single cell a metre long —
dynein provides the transport from the toe back to the nucleus.
Earlier this year, Kiyomitsu, working in Cheeseman's lab, saw that during the metaphase cell cycle step a dividing cell uses the
motor protein
dynein and two signals to perfectly align the cell's mitotic spindle structure in the middle of the cell.
By combining many images of individual
motors, we were able to sharpen up our picture of the
dynein and build up a dynamic idea of how it moved.
Other
motor proteins, called kinesins and myosins, are much smaller and have specific functions, but
dynein can turn its hand to a lot of different of functions,» Professor Knight said.
Dr Stan Burgess, at the University of Leeds» School of Molecular and Cellular Biology, who led the research team, said: «
Dynein has two identical
motors tied together and it moves along a molecular track called a microtubule.
Functional analysis of human microtubule - based
motor proteins, the kinesins and
dyneins, in mitosis / cytokinesis using RNA interference.
Williams focused on
dynein light chains, which were thought to bridge Fyn kinase and other cargo to the molecular
motor dynein.
During G1 phase, basal migration of the nucleus requires the plus - end - directed microtubule
motor Kif1A, while during G2 phase, apical migration requires the minus - end - directed
motor dynein.
Delanoue R., Herpers, B., Davis, I. and Rabouille, C. Drosophila Squid - hnRNP helps
dynein switch from a gurken mRNA transport
motor to an ultrastructural static anchor in sponge bodies Developmental Cell 13:523 (2007)
The investigators showed that kinesin movement corresponds to traffic from the center of a nerve cell to its axonal tips — and a different
motor in the squid's cytoplasm, which was subsequently identified as
dynein, travels in the opposite direction.