«Previously,
dynein movement had only been tracked by attaching fluorescent molecules to the proteins and observing the fluorescence using very powerful light microscopes.
Not exact matches
The axoneme's
movement is accomplished via rows of motor proteins called
dyneins that are attached along the microtubules and exert force on them so the microtubules «slide» past each other, which then causes the entire axoneme and sperm tail to bend and move.
At the molecular level, individual
dynein molecules whose shapes are stabilized by prestress were found to have areas of increased rigidity around their ATP binding sites, which resist deformation by incoming energy from ATP and instead translate that force into the
dynein molecule's characteristic
movement.
They used this novel simulation approach to build a model of a sperm cell that demonstrates cellular
movement from individual
dynein protein molecules in the tail all the way up to the whole cell, allowing them to observe how changes at the atomic level are reflected in larger - scale structures.
At Berkeley, Yildiz expanded his studies on the
movement of
dynein motors and also branched out in a new direction.
The investigators showed that kinesin
movement corresponds to traffic from the center of a nerve cell to its axonal tips — and a different motor in the squid's cytoplasm, which was subsequently identified as
dynein, travels in the opposite direction.