Apoptosis and necrosis were quantified using immunofluorescence assays; bioconjugate - treated cells were
early apoptotic after 4 hours, proving effective initiation of programmed cell death.
«Flow cytometry identified a large population of
apoptotic melanoma cells, and single - cell electrophoresis indicated that mensacarcin causes genetic instability, a hallmark of
early apoptosis,» Loesgen said.