The early cardiac markers and transcription factors (Gata4, Mef2c, Hind I, Nkx2 - 5 and beta - Mhc) were examined in iPS cell lines 11.1 and 4.1 during the cardiac differentiation process (differentiation day 0, day 3, day 6 and day 9).
Interestingly, cells from both iPS cell lines expressed
early cardiac differentiation
markers and transcription factors, implicating that there could be a lack in functional cardiomyocyte maturation in line 4.1.