Briefly, full - length secreted molecules and the entire
ectodomains of membrane - embedded proteins were identified using transmembrane [28], GPI - anchor [29], and signal peptide [30] prediction software.
FMP2.1 is a lyophilized preparation of
the ectodomain of the 3D7 clone of P. falciparum AMA1 [21].
Not exact matches
Increasing evidence suggests that the synaptic functions
of the amyloid precursor protein (APP), that is key to Alzheimer (AD) pathogenesis, may be carried out by its secreted
ectodomain.
Mechanistic Contributions
of the p10 FAST Protein
Ectodomains to Membrane Fusion and Syncytiogenesis.
This resource
of extracellular P. falciparum merozoite proteins, which now contains 62 full - length
ectodomains, will be a valuable tool in elucidating the function
of these proteins during the blood stages
of infection, and facilitate the comparative assessment
of blood stage vaccine candidates.
Consequently, the functional characterization
of extracellular parasite proteins has typically been restricted to smaller subfragments that can be expressed rather than the full - length protein or entire
ectodomain, which is more likely to be representative
of the native protein.
A band consistent with the entire
ectodomain was observed by immunoblotting for the majority
of the proteins and their expression levels were quantified.