Sentences with phrase «ectopic expression»

As shown in Fig. 4B, suppressive ability of miR - 7 on both transmigration through endothelial cells and Matrigel was significantly attenuated by ectopic expression of KLF4, suggesting that miR - 7 suppresses invasive ability of CSCs by blocking KLF4 expression.

Furthermore, the growth rate is delayed by ectopic expression of DDX3 (see Fig. 1B).

To evaluate whether KLF4 regulates the self - renewal of CSCs, we conducted fluorescence - activated cell sorting (FACS) analysis of 231BrM cells with or without ectopic expression of KLF4 followed by sorting the cells using the CSCs markers (CD24, CD44, and ESA).

Since we observed robust expression of SOX2 and modest expression of c - MYC and KLF4 in our human NSC cultures, we tested if ectopic expression of OCT3 / 4 per se would be sufficient to convert them from multipotent to pluripotent stem cells.

Results: Fibroblast growth factor 2 (FGF2) treatment reduced expression of β - cell markers, (INS, MAFB, SLC2A2, SLC30A8, and GCK) and activated ectopic expression of MYC, HES1, SOX9, and NEUROG3.

Here we show that human NSCs indeed can be reprogrammed into iPS cells by ectopic expression of OCT3 / 4 and KLF4.

We also found that overexpression of miR7 in 231BrM cells decreased the tumor size by around 30 %, and this effect was blocked by ectopic expression of KLF4, indicating that miR7 does affect primary tumor but not to the level of metastasis (Supplementary Fig.

Dr. Guo says: «Muffs and beard in chicken is caused by a structural variation that consists of three duplicated regions on GGA27 and results in the ectopic expression of HOXB8 in facial skin.

Importantly, ectopic expression of miR - 7 significantly blocked the brain metastasis but not bone metastasis in our animal model.

As shown in Fig. 5A, ectopic expression of KLF4 indeed significantly increased CSCs population in 231BrM cells.

In fact, we found that miR - 7 was downregulated in mammosphere, a hallmark of self - renewal of CSCs, and that ectopic expression of miR - 7 significantly reduced the ability in mammosphere formation.

A, CSCs isolated from 231BrM and MB231 with the ectopic expression of miR7 and miR7 - LNA were seeded on top of mouse brain endothelial cells or Matrigel.

A well - defined cell type that has been widely used to identify the consequences of ablation or ectopic expression of a gene of interest.

Ectopic expression of retinoic acid early inducible - 1 gene (RAE - 1) permits natural killer cell - mediated rejection of a MHC class I - bearing tumor in vivo.

But how can we induce astrocyte to NSC conversion without the ectopic expression of neurogenic transcription factors?

Moreover; the ectopic expression of GRM1 mediated melanoma agenesis is independent of the genotype of either BRAF or NRAS [39].

It has been observed that ectopic expression of metabotropic glutamate receptor 1 (GRM1) in melanocytes is sufficient to induce spontaneous melanoma development in vivo and transformation in vitro [37, 38].

Additionally, Western blot analysis indicated that the expression level of p21waf1 / cip1 gene was also enhanced by the ectopic expression of DDX3 in HuH - 7, HeLa, and 293T cells (Fig. 2B).

Ectopic expression of DDX3 inhibits cell growth.

They can be reprogrammed by ectopic expression of two factors (Oct4 and Klf4) or even one factor (Oct4) alone [14], [15], providing a unique tool to analyze reprogramming events at a molecular level.

Ectopic expression of dPrx5 in mitochondria but not nucleus or cytosol partially extended longevity under normal or oxidative stress conditions while complete restoration of life span occurred when all three forms of dPrx5 were expressed from the wild type dPrx5 transgene.

Ectopic Expression of Rubisco Subunits in Maize Mesophyll Cells Does Not Overcome Barriers to Cell Type - Specific Accumulation