Sentences with phrase «edited clonal»

Pluripotency was maintained in all edited clonal lines, as evidenced by the persistent expression of the three pluripotency markers.

Single - cell seeding of edited hiPS cells in this system results in high survival of pluripotent, edited clones, yielding a diverse set of edited clonal lines.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Kit:

The karyotypes of five edited clonal cell lines were analyzed.

Later, this edited clonal cell line can be differentiated into a cell type relevant to the disease under study (e.g., neurons, as depicted in Figure 1).

Not exact matches

Traditionally, establishing clonal populations from edited hiPS cells grown and passaged as colonies is inefficient and time consuming; often, it results in cell death or premature differentiation.

Individual, edited (CD81 knockout) hiPS cells were expanded into clonal lines and analyzed for expression of CD81 and three pluripotency markers via flow cytometry using antibodies against CD81, Oct ‑ 4, TRA ‑ 1 ‑ 60, and SSEA - 4.

hiPS cell clones that have been edited via gesicles maintain a stable karyotype after editing and clonal expansion.

The Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System provides an efficient and effective method to generate clonal lines of edited hiPS cells.

Promoting survival and proliferation at the single - cell level is critical for expansion of any clonal colonies containing the mutation of interest generated by the gene editing process.