Sentences with phrase «editing method generating»

Not exact matches

We have developed a method called the mutagenic chain reaction (MCR), which is based on the CRISPR / Cas9 genome - editing system for generating autocatalytic mutations, to produce homozygous loss - of - function mutations.

We'll have a post that goes into the mouse genome editing process in a bit more detail in the coming weeks, but, in this post, we will outline a simple method for selecting the guide RNA, validating its efficacy in vitro, and using it in mouse embryos to generate gene modified mouse lines.

In vitro screening is a simple and fast method for validating your selected gRNA's, and has the bonus benefit of supplying you with primers for validating your edit in mice once they have been generated (more on that later).

The Cellartis iPSC CRISPR / Cas9 Gesicle and Single - Cell Cloning System provides an efficient and effective method to generate clonal lines of edited hiPS cells.

Ask successful authors what single method guarantees book sales, and the answer isn't «great editing» or a «knock - em - dead cover», but the power of their email list to enthuse readers, make their books visible and generate reviews and sales.