Sentences with phrase «effectors for»

For the past 20 years, he has identified and characterized several important naturally occurring TAL effectors in the rice pathogen Xanthomonas oryzae for their disease promoting ability, and most recently he has helped harness the disease - causing TAL effectors for targeted gene editing.

The attraction of large - scale compound screening for life science researchers lies mainly in the technical challenge of assay design and the use of small - molecule effectors for hypothesis - driven research.

Calmodulin (CaM) is a major effector for the intracellular actions of Ca2 + in nearly all cell types.

Not exact matches

It includes BPA's robotic platform provided with dedicated handling parts to match your specific application, including our patented SmartTrak system for high speed bag pre-collation and end - effector.

These are receptors on immune cells, which control for example effector T - cells by dampening their activation if damage to healthy cells is imminent.

For instance, in autoimmune disease, effector T cells are overly activated and cause damage to body.

They systematically deleted genes for secreted effector proteins — molecules that the parasite injects into a host cell to modulate the immune system during infection — and injected the altered parasites into mice with aggressive ovarian cancer.

Single - cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.

As the end products and biological effectors of gene expression, proteins are crucial for improving our understanding of human biology, developing new and better drugs, and advancing precision medicine.

Further insight into the basic details of CRISPR - Cas structure, functions, and biology — and characterization of new Cas effector proteins in particular — is crucial for optimizing and further expanding the diverse applications of CRISPR - Cas systems.

As well as innovative tools for basic research, CRISPR - associated effector complexes will be instrumental for developing the next generation of antiviral prophylactics and therapeutics.

He and his colleagues believe the effectors offer good targets for breeding or engineering disease resistance varieties.

The phenotype of NP cells generated ex vivo (Figure S8) closely resembles that of central memory CD4 + T cells found in vivo, which persist for years in secondary lymphoid organs and can differentiate into effector memory CD4 + T cells [45].

She demonstrated, for the first time, that the pathogenesis of type 1 diabetes in NOD mice correlated with an enhanced pathogenicity of T effector cells and a decreased suppressive activity of Tregs.

TNF is one of the most potent effector cytokines in the pathogenesis of autoimmune disorders, and blockade of TNF with anti-TNF antibodies is an effective treatment for many patients with IBD.

The findings suggest an alternate indirect effector pathway that requires processing and presentation of the donor H - Y antigen by recipient endothelium and have implications for both transplantation and autoimmune disease.

Removing one immune effector element only reveals the presence of an ancillary pathway for allograft rejection.

The team additionally identified a new effector pathway for alloreactive T cells.

The HZI will develop a vaccination protocol for mucosal administration based on three novel strategies: (i) development and optimization of a vaccination protocol in which parenterally - primed T and B cells are subsequently pulled into the mucosa by the local delivery of the cognate antigen to the requested effector site, (ii) testing the co-administration of antigens with novel mucosal adjuvants using different mucosal immunisation routes and schedules, and (iii) testing various nanoparticles co-administered with different immunomodulators for their ability to generate both systemic and mucosal immune responses following transcutaneous / trans - follicular vaccination.

Therefore, in both mice and non-human primates, HO - 1 inducers delivered locally inhibited effector T - cells in an antigen - specific manner, paving the way for repositioning these drugs for the treatment of immune - mediated diseases.

Hunt for ceramide trafficking machinery and effectors in cells.

Constitutive cytokine mRNAs mark natural killer (NK) and NK T cells poised for rapid effector function.

Six weeks postinfection, splenocytes were analyzed for gp33 - LCMV — specific total CD8 + T cells (B) or short - lived effector cells and memory precursor effector cells (C).

Of special interest were ICOS, whose «role in effector T cell responses and anti-tumor immune responses is now well established,» and CD40, which is «important for the maturation of antigen - presenting cells and for enabling T cell co-stimulation.»

MEKK4 is an effector of the embryonic TRAF4 for JNK activation.

Consistent with prior reports on effector cells (35), we observed that, among CD8 + T cells specific for the dominant epitope of LCMV (GP33), the percentages of short - lived effector cells (KLRG1 + CD127 −) were increased, and the percentages of memory precursor cells (KLRG1 + CD127 +) were decreased in the peripheral blood of DGKζ − / −, Cbl - b − / −, and DKO mice relative to WT mice after acute infection (Fig. 5A).

Science magazine heralded these transcription activator - like effector nuclease proteins, known as TALENS, as a major scientific breakthrough in 2012, nicknaming them «genomic cruise missiles» for their ability to allow researchers to target specific locations with great efficiency.

«Inhibition of mTOR, or its downstream effectors, may represent a novel chemopreventive strategy for this type of aggressive HCC,» she said.

As mRNA isolation is simple and since DNA microarrays are a proven genomics reagent for monitoring differential gene expression, a genome - wide expression analysis may be the fastest and most efficient method for identifying additional candidate effectors of colony queen number.

They will also prepare a list of the genetic codes of the fungus — the so - called effectors — that are responsible for the ability of the fungus to adapt to new wheat varieties.

In addition to being MHC II negative, MSC do not appear to express the co-stimulatory molecules CD40, CD40L, CD80 or CD86 required for effector T cell induction 104, 105.

In order to find these elusive effector proteins, Klemke and Wang are using a technique that Klemke has developed over the last decade for looking specifically at the leading edge of a migratory cell.

The variety of new tools available for genetic manipulation now include lentiviral - based gene delivery, and gene editing using CRISPR / Cas9, zinc finger nucleases (ZFNs) or transcription activator - like effector nucleases (TALENs).

Yang Y, Lu Y, Espejo A, Wu J, Xu W, Liang S, Bedford MT (2010) TDRD3 is an effector molecule for arginine - methylated histone marks.

This protocol describes a system for efficient genome editing in hPSCs using engineered transcription activator - like effector nucleases (TALENs) or clustered regularly interspaced short palindromic repeat (CRISPR) technology.

She is registred to the National Order of Biologists in the province of Palermo; collaboration in research project from 2012 to 2015 at the Department of Biopathology and Biotechnology, University of Palermo, focusing the study on the identification of molecules capable to modulate intracellular metabolic pathways for the prevention and treatment of infectious, tumor and degenerative disease, in collaboration with Prof. Angela Santoni, University of Rome; collaboration in research project in 2011 at the hospital «Villa Sofia Cervello» of Palermo to study methods can cure the genetic defect that causes thalassemia through genetic engineering; she studies different mechanisms of the differentiation and the activation of human gammadelta T cells as effector cells of the immune response against cancer and infectious diseases; she investigates about the identification and development of biomarkers of resistance and susceptibility to Mycobacterium tuberculosis infection; Valentina Orlando has published 13 papers in peer reviewed journals and 3 comunications at national and international congress.

Effector and suppressor roles for LFA - 1 during the development of experimental autoimmune encephalomyelitis.

Identifying any Hymenoptera - specific immune pathways or effectors that may exist is also of key importance for understanding the biology of host - pathogen interactions that may be relevant for such challenges as colony collapse disorder [42]--[44].

Scatterplot (with jitter of overlapping points) showing fold - change for AMPs, other effectors, recognition, signaling, proteases, and non-immune genes.

Because of their ability to differentiate into various effector T subsets, Th1, Th2, Th17, and Tregs, CD4 + T cells are particularly important in the initiation of GVHD in mice and have been considered potential targets for the treatment and prevention of GVHD in the clinic.

Helicobacter pylori effector protein CagA activates the signal transduction and activator of transcription 3 (STAT3) signaling pathway: a potential mechanism for gastric cancer development.

This correspondence makes it possible to rapidly identify TAL effector targets, to engineer novel TAL effectors with custom assortments of repeats to bind DNA sequences of choice, and even to customize genes for TAL effector activation (or prevent it!).

Major areas of activity in our lab include 1) identification and characterization of Sgenes, 2) genomic analyses to identify the diversity of TAL effectors in pathogen populations and understand their evolution, and 3) structural and biochemical studies to better harness the unique properties of these proteins for applications such as targeted gene regulation and genome editing.

To examine the functional role of Smad1 and Smad5 in GDF5 - induced neurite length, primary cultures were transfected by microporation with siRNAs targeting the co-Smad4 (required for nuclear translocation of Smad1 / 5 transcriptional complex [33]-RRB- or the Smad1 and Smad5 effectors proteins, which are known targets of miR - 181a (Table 2).

We are currently investigating the details of the signaling cascades involved in these phenomena, as well as attempting to identify cytoplasmic binding partners for NG2 which may serve as effector molecules for activation of downstream signaling events.

Whereas when an effector is detected inside the plant cell, the plant knows for sure it is being attacked and it activates ETI, a very strong immune response,» said co-author Greg Martin, the Boyce Schulze Downey Professor at BTI.

For the candidate gene in the region, RSPO3, we demonstrated expression in key liver - resident effector cells, such as human and murine cholangiocytes and human hepatic stellate cells.

Background: Many pathogens secrete effector molecules to subvert host immune responses, to acquire nutrients, and / or to prepare host cells for invasion.

136/6: 15 Trans - ethnic meta - analysis reveals novel loci and effector genes for kidney function in diverse populations.

Both effector strategies can also be used for an inducible targeting strategy.