Sentences with phrase «efficiency targeted gene»

Not exact matches

In an effort to combine the respective advantages of the AAV and CRISPR - based approaches, several groups have recently worked to enhance the efficiency of AAV - based gene editing via the introduction of a double strand break by using a targeted nuclease.

Because of their unique properties such as monodispersity, large surface area and high drug loading efficiency, silica nanoparticles (SiNPs) have been developed for a vast array of biomedical uses such as optical imaging, cancer therapy, targeted drug delivery and controlled drug release for genes and proteins.

We next tested the efficiency of Cas9 / gRNAs disrupting four genes simultaneously by co-transfecting pooled Cas9 / gRNAs targeting MSTN, BLG, PrP and NUP genes.

The plasmids targeting each gene were respectively transfected into goat fibroblasts, and their genome modification efficiency was determined at day 3 using restriction fragment length polymorphism (RFLP) assay (see Methods).

Comparing this approach to the classic CRISPR / Cas9 system designed to cleave the DNA, the Gladstone team found that CRISPRi is actually more effective, silencing the target gene in more than 95 percent of cells, compared with the 60 percent to 70 percent efficiency of CRISPR / Cas9.

But CRISPR's speed, efficiency and ability to target multiple genes simultaneously are making it the new standard for knockouts in mice, zebrafish and many other species.

Using ∼ 2000 Plasmodium falciparum genome sequences, we investigated the genetic diversity of eleven gene - targets of promising antimalarial compounds and assessed their potential efficiency across malaria endemic regions.

Genes regulating the cerebral levels of important neurotransmitters (dopamine, serotonin, GABA, etc.) or signal transmission efficiency (neurotransmitter receptors and genes) have been targeted in association studies of major psychiatric disorders such as schizophrenia, bipolar disorders, attention deficit / hyperactivity disorder (ADHD), and autism [84], as well as of personality traits Genes regulating the cerebral levels of important neurotransmitters (dopamine, serotonin, GABA, etc.) or signal transmission efficiency (neurotransmitter receptors and genes) have been targeted in association studies of major psychiatric disorders such as schizophrenia, bipolar disorders, attention deficit / hyperactivity disorder (ADHD), and autism [84], as well as of personality traits genes) have been targeted in association studies of major psychiatric disorders such as schizophrenia, bipolar disorders, attention deficit / hyperactivity disorder (ADHD), and autism [84], as well as of personality traits [85].