Sentences with phrase «efflux transporters»

Sanny S. W. Chung, Xiangyuan Wang, Debra J. Wolgemuth; Prolonged Oral Administration of a Pan-Retinoic Acid Receptor Antagonist Inhibits Spermatogenesis in Mice With a Rapid Recovery and Changes in the Expression of Influx and Efflux Transporters, Endocrinology, Volume 157, Issue 4, 1 April 2016, Pages 1601 — 1612, https://doi.org/10.1210/en.2015-1675

Second, our current study suggests that after the long - term drug administration in mice, the more rapid recovery may also involve the interplay of ABC efflux transporters and SLC influx transporters in the testes.

A, The expression of several ABC efflux transporters, including Abcb1a, Abcb9, Abcc5, and Abcc10, were significantly up - regulated in testes after long - term drug treatment as compared with the control.

Interestingly, we found that at the protein level 2 efflux transporters, namely ABCB1 and ABCC10, were up - regulated and now are also expressed in germ cells, expression that was barely detectable, if at all, in controls.

To begin to explore the cellular mechanisms that might be responsible for this response, histological analysis revealed less severe disruption of spermatogenesis and gene expression studies suggested that an interplay of ATP - binding cassette (ABC) efflux transporters and solute carrier (SLC) influx transporters in the testicular cells might be involved.

Indeed, we found that long - term drug treatments were associated with increased expression of several ABC efflux transporters and influx transporters.

For example, up - regulated influx transporters (OCT3 and OCTN1) localized at the apical membranes of Sertoli cells could mediate the transport of the drug compound 9 from the lumen into the basolateral compartment of Sertoli cells, where the up - regulated efflux transporters (ABCB1 / p - glycoprotein, ABCB8, ABCB9, ABCC5, and ABCC10) could then transport the drug out of Sertoli cells against a concentration gradient, resulting in enhanced efficiency of recovery upon cessation of treatment.

Prolonged oral administration of a pan-retinoic acid receptor antagonist inhibits spermatogenesis in mice with a rapid recovery and changes in the expression of influx and efflux transporters.

Importantly, cisplatin toxicity and Pgp efflux transporter activity measured on - chip more closely mimic the in vivo responses than results obtained with cells maintained under conventional culture conditions.