The new finding brings a measure of closure to a story that first rocked the science world in February 2004, when Hwang and colleagues at Seoul National University announced they had cloned a female donor's cell by transferring its nucleus into one of
her egg cells stripped of its nucleus in a procedure known as somatic cell nuclear transfer (SCNT), and harvested embryonic stem cells from the resulting fusion.
Using a process called somatic cell nuclear transfer (SCNT), a team from Oregon Health & Science University (O.H.S.U.) in Portland implanted the contents of individual skin cells from adult male rhesus macaques into each of 304 macaque
egg cells stripped of their genetic material.
The scientists starved the cells into quiescence, then transferred their DNA - carrying nuclei into
egg cells stripped of their own nuclei.
Not exact matches
It is far more likely, however, that the
egg -
cell cytoplasm with its
stripping factor will reprogram all the genetic material including the alterations made in the donor nucleus that were intended to prevent the creation of the zygote.
For instance, the
egg -
cell cytoplasm
strips off all of the many epigenetic factors which differentiate a genetically restricted donor skin
cell from a totipotent zygote.
The
egg cell must then reprogram the nucleus's DNA, basically
stripping the body
cell of its identity and returning it to an embryonic state.
In theory, Puntung could be cloned, taking a nucleus from one of her
cells and injecting it into an
egg stripped of its own DNA.
Noah was created by fusing skin
cells from a gaur — a rare species of wild ox that lives mostly in India — that had died in 1993 with cow
eggs stripped of their nuclei.