Taking up this challenge, a team at Rudolf Jaenisch's lab at the Massachusetts Institute of Technology transferred the nuclei of olfactory neurons into
egg cells whose nuclei had been removed.
The researchers then transferred nuclei from nearly 1900 of the cultured cells into
egg cells whose nuclei had been removed, eventually producing six calves.
Experts take the cell nucleus of one human
egg cell whose mitochondria have a defect and place it in an egg cell with «healthy» mitochondria.
Instead, they rely on transplanting the genetic information from a specialized cell into an unfertilized
egg cell whose genetic information has been destroyed or physically removed.
Not exact matches
The
egg then grew into an early - stage embryo
whose stem
cells, a genetic copy of the original, were then harvested.
In this process, the nucleus of a donor adult
cell is transferred to an
egg whose nucleus, and thus its genetic material, has been removed.
Combining an
egg's genetic leftovers with donor
cells may be a way to double the number of
eggs available for IVF in women
whose ovarian reserve is running low
They selected
cells that had taken up the DNA and placed them in contact with cow
eggs whose nuclei had been removed.
One group was born from
eggs whose nuclei had been replaced with genetic matter from a type of
cell called a cumulus
cell that surrounds the ovaries.
The paper doesn't include any genetic analysis of the final
eggs that confirms they are healthy, notes Mitinori Saitou, a stem
cell biologist at Kyoto University in Japan
whose team developed methods to create mouse
egg cells from embryonic or reprogrammed stem
cells.
Like Dolly's creators in Scotland, Don Wolf of Portland's Oregon Health Sciences University and his colleagues transferred the nuclei from donor
cells to
eggs whose own DNA had been removed.
Dolly was created using nuclear transfer, a technique in which an intact donor
cell is fused with an
egg whose nucleus had been removed.