Cells and synapses reconstructed from serial block face
electron microscopy data.
Norma is used to interpret
electron microscopy data using atomic scale resolution structural models and perform flexible fitting on new cases without encountering major technical difficulties.
Not exact matches
Over the last half - century, protein structure
data from imaging techniques like X-ray diffraction and
electron microscopy has mounted, and protein structure databases store at - the - ready information on sequencing and structure.
Therefore, before publication, large
data sets (including microarray
data, protein or DNA sequences, atomic coordinates or
electron microscopy maps for molecular and macromolecular structures, and climate
data) must be deposited in an approved database and an accession number or a specific access address must be included in the published paper.
One mission of the Facility is to establish user - friendly workflows ranging from sample preparation to image acquisition, image processing, and long - term
data storage, especially for imaging modalities generating very big datasets or files, such as slide - scanning, high - content screening, and 3 - D scanning
electron microscopy.
Acquiring images using modern techniques such as light sheet fluorescence, confocal, or
electron microscopy creates a significant
data stream.
«This method allowed us to drastically expand the previously available
data, acquired through scanning
electron microscopy; it also made possible to characterize surface patterns directly, not based upon analysis of metal replicas.
This work, with the assistance of soil scientists at the University of KwaZulu - Natal, has involved a suite of techniques, including x-ray fluorescence (to provide quantitative
data on minor and trace element composition), x-ray diffraction (to reveal crystal structure and parent rock types of paint ingredients), and environmental scanning
electron microscopy (to yield qualitative
data on elements present).
It took more than a year to analyse the
data the scientists had obtained using the cryo -
electron microscopy facility at Maastricht University.
Mercer says the team has since done
electron microscopy experiments that show the virus moving into the cell through membrane indentations, although these
data were not part of the published study.
Korenberg's team is developing a 3D coordinate system to align various types of neuroimaging
data in the macaque brain, from whole - brain MRI connectivity to single - cell confocal
data and, for some areas, subcellular resolution with
electron microscopy.
The current focus of his work is finding ways to apply new methods to multiple modes of
data collection, from X-ray diffraction to cryo -
electron microscopy, X-ray free
electron laser (XFEL) technology, and
electron diffraction.
Recently, Pyle used this mathematical approach to create libraries of discrete RNA conformations that can be used to build RNA structures using
electron density
data and, in the future,
data from cryoelectron
microscopy.
The image is generated from reconstructed
data from serial block face scanning
electron microscopy, showing the cells, their flagella (red and purple) and internal organelles.
Correlative light and
electron microscopy (CLEM) is an imaging technique that combines
electron microscopy (EM) with fluorescence localization
data.
The Fraser team borrows techniques from X-ray crystallography, NMR, and cryo -
electron microscopy and develops new methods to gather and to analyze the
data.
As a member of the Numerical Algorithms group, he develops new methods for analysis of biological
data, with particular focus on cryo -
electron microscopy.