To further prove the specificity of the binding of STAT6 to the COX - 2 promoter sequence,
electrophoretic mobility shift assays (EMSA) were performed utilizing a 30 bp oligo from the COX - 2 promoter sequence encompassing the STAT6 binding site as a probe.
STAT6 DNA - binding specificity was confirmed by
electrophoretic mobility shift assay.
Neuronal cells express a pp60c - src variant that displays an altered
electrophoretic mobility and a different V8 peptide pattern relative to pp60c - src expressed in tissues of non-neuronal origin.
The zeta potentials of the samples were obtained from laser Doppler electrophoresis, converting
electrophoretic mobilities to zeta potentials.