Sentences with word «electroporation»

Additionally, the authors tested for cellular specificity by using as a convenient experimental framework in utero electroporation of a plasmid that allowed co-expression of the PLE enzyme and of a fluorescent tag (mCherry).

A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization.

HV cells constitutively expressing the LacZ gene were generated by electroporation with a vector containing a CAG driven β - Geo cDNA followed by selection in G418 (150 µg / ml) for 2 wk.

Researchers commonly use electroporation, a technique that uses an electric pulse to momentarily create pores that allow the Cas9 protein and guide RNA complex to slip inside cells in a dish.

The W / O droplet electroporation technique can contribute to further biomedical innovation in high - throughput screening with a large number of samples for applications in regenerative medicine and gene therapy.»

More importantly, Neon provides highly efficient CRISPR / Cas9 gene knock - in with our newly developed all - in - one electroporation method, which is ideal for next generation cell therapy.

The article, «Retrobulbar Inflammation, Adipogenesis and Acute Orbital Congestion in a Preclinical Female Mouse Model of Graves» Orbitopathy Induced by Thyrotropin Receptor Plasmid - in Vivo Electroporation,» will be published in the September issue of Endocrinology.

Associate Professor Rika Numano explained, «The W / O droplet electroporation technique has several advantages over conventional transfection techniques: small cell number required, as low as 1000 cells; small amount of DNA required, which makes it applicable for various cell types including neural cells; and changeable genome DNA.

Tissues targeted (as reported in animals): Primarily blood, immune, and stem cells removed from the body; localized electroporation to skin

Callosal axon branching (green, contralateral side) labeled by in utero cortical electroporation at E15, examined at P21 (from Julien Courchet).

Systemic antitumor effect and clinical response in a phase 2 trial of intratumoral electroporation of plasmid interleukin - 12 in patients with advanced melanoma.

Through a process called electroporation, bacterial cell membranes are selectively damaged.

H. Efficient CRISPR / Cas9 - Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.

Following electroporation into R26 BirA cells, a cell line that expresses bacterial BirA ligase from the ROSA26 locus, hygromycin resistant clones (200 µg / ml) were expanded for Southern analysis to identify correct targeting events.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

Using Pulse Electric Field Processing (PEF) the patent protected E-FLO Electroporation System provides significant product improvement in chip crunch, taste and texture as well as reduction in acrylamide formation and oil content.

This new electroporation delivered the genome - editing molecular for generating mutant animals.

«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water - in - oil droplet.»

However, most commercial electroporation - based transfection methods require the use of specialized pulse generators to produce short electrical pulses at high voltage.

There have been improvements in W / O - droplet electroporation electrodes for use in disposable 96 - well plates for concurrent performance (Fig. 2).

In addition, the size of the droplet is quite small compared with that in conventional bulk electroporation, resulting in the use of fewer materials in high - throughput analysis.»

They then used a method known as electroporation, in which cells are briefly exposed to an electrical field that makes their membranes more permeable, to quickly deliver these RNPs to the interior of the cells.

These are low passage wild type parental clones suitable for a single electroporation and germline transmission.

Electroporation increases the efficiency of model creation.

For both BayGenomics parental ES cell line E14Tg2a.4 (also used for Sanger (SIGTR) lines) and Soriano parental ES cell line AK7, ultra low passage cells suitable for multiple electroporations and germline transmission are available.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Kit:

This entire construct was inserted into the Gt (ROSA) 26Sor locus via electroporation of 129X1 / SvJ - derived JM - 1 embryonic stem (ES) cells.

When tested in retinal electroporation studies, DsRed reporter expression was induced in ~ 76 % of GFP + cells, and 100 % of DsRed + cells also expressed GFP.

Important reports from the Weiner lab include the first DNA vaccine studied for HIV as well as for cancer immune therapy of cutaneous T cell lymphoma, the early development of DNA encoded genetic adjuvants, including IL - 12, advances in gene optimization, and advances in electroporation technologies resulting in improved gene delivery.

Though speaking of which, I should go ask if electroporation counts for the «electrical shocking of an organism» badge...

For a typical library, Addgene usually does four to eight electroporation reactions using a total of ~ 400ng of library DNA.

Because pronuclear injections and ES cell electroporations utilize unique DNA sequences that recombine into the mouse genome, the principal investigator needs to provide the GEM Facility with an approval number from the Institutional Biosafety Committee (IBC) prior to beginning these experiments.

This causes what's called reversible electroporation, where these cells will transiently increase their ability to be permeable to foreign compounds, and so you're able to get the cancer drugs into the tumors and kill them.

HV cells overexpressing Nanog were generated by electroporation with a vector containing the Nanog cDNA under the control of a CAG promoter and upstream of IRES Puro cassette followed by selection in puromycin (2 µg / ml) for 2 wk.

The amenability of the chick embryo to manipulation and imaging, combined with the powerful technique of in vivo electroporation and the strong similarities of muscle formation in birds and mammals provide a unique paradigm to characterize this process in amniotes.

Expansion of embryonic and adult neural stem cells by in utero electroporation or viral stereotaxic injection.

Primarily blood, immune, and stem cells removed from the body; localized electroporation to skin

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).

With intratumoral electroporation, tumor cell pores are opened when a mild electrical current is introduced directly through an electrode.

Highly - engineered monoclonal antibody sequences are encoded in a DNA plasmid and delivered directly into cells of the body using electroporation, resulting in protective monoclonal antibodies «manufactured» in the body — versus recombinant proteins manufactured and purified in vitro.

Now, researchers at the Department of Environmental and Life Sciences and Electronics - Inspired Interdisciplinary Research Institute (EIIRIS) in the Toyohashi University of Technology have developed a novel gene - transfection method: water - in - oil (W / O) droplet electroporation with sufficient transfection efficiency and cell viability.

Interim phase II results [12] of first treatment with electroporation of IL - 12 in 28 patients with advanced melanoma, after 24 weeks of treatment, reported at the American Society of Clinical Oncology 2014 Annual Meeting, revealed a 32.2 % objective response rate (ORR; the primary endpoint), with a CR in 10.7 %.